Združeni učinak anestetika sevoflurana i ionizirajućeg zračenja na primarna oštećenja DNA u miša

master's thesis

• Title (english): The combined effect of anaesthetic sevoflurane and ionising radiation on primary DNA damage in mice
• Author: Sanja Halovanić
• Mentor: Vesna Benković (mentor)
• Committee member: Vesna Benković (predsjednik povjerenstva)
• Committee member: Zora Popović (član povjerenstva)
• Committee member: Zdravko Dolenec (član povjerenstva)
• Committee member: Vesna Petrović Peroković (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2012-12-13, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Sevofluran, fluorometil-heksafluoroizopropil-eter, je inhalacijski anestetik, a slaba topljivost i neiritabilnost omogućuju mu široku primjenu u uvodu i održavanju opće anestezije. Ionizirajuće zračenje ima široku primjenu u medicini (u radioterapiji). Cilj istraživanja je utvrditi i usporediti međudjelovanja γ-zračenja (60Co) u klinički relevantnoj dozi (1 Gy) i anestetika sevoflurana (2,4%, 50:50) na modelu miša soja Swiss albino, s naglaskom na proučavanje stupnja osjetljivosti različitih vrsta tkiva (mozga, jetre i bubrega) i leukocita periferne krvi. Za procjenu primarnih oštećenja DNA, primijenjena je metoda alkalnog komet testa neposredno nakon, te 2, 6 i 24 h od izlaganja anesteziji i zračenju. Najveći genotoksični učinak izmjeren je u stanicama bubrega i mozga 2 sata, u krvi 6 sati, a u jetri 24 sata nakon tretmana životinja u odnosu na kontrolnu skupinu. Za ispitivanje razlika u količini popravka između krvi i različitih tkiva, izračunat je stanični indeks popravka (CRI – od engl. cellular DNA repair index), koji je pokazao različitu osjetljivost organa.

Abstract (english)

Sevoflurane (hexafluoroisopropyl fluoromethyl ether) is inhalation anaesthetic which low solubility and non-irritability, provides its wide application in induction and maintenance of general anesthesia. Ionising radiation is widely used in medicine (radiotherapy). The aim of the study was to determine and compare the interaction of γ-radiation (60Co) in a clinically relevant dose (1 Gy) and anaesthetic sevoflurane (2.4%, 50:50) in the model of Swiss albino mices, with an emphasis on the study of the degree of sensitivity of different types of tissue (brain, liver and kidneys), and peripheral blood leukocytes. For the assessment of primary DNA damage, the method of alkaline comet assay was applied immediately after, and 2, 6 and 24 h of exposure to radiation and anaesthesia. The strongest genotoxic effect was measured in kidney and brain cells 2 hours, in the blood 6 hours, and in the liver 24 hours after the treatment of the animals as compared to a control group. To test the difference in the amount of repair between blood and various tissues, cellular DNA repair index (CRI) was calculated, which showed different sensitivity of organs.

• Keywords: genotoksičnost; komet test; leukociti; jetra; bubreg; mozak; stanični index popravka DNA
• Keywords (english): genotoxicity; comet assay; leukocytes; liver; kidney; brain; cellular DNA repair index
• Language: croatian
• URN:NBN: urn:nbn:hr:217:369599
• Study programme: Title: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-02 08:00:59