Utjecaj perinatalne primjene agonista serotonina na imunološke i hematološke parametre u odraslih štakora

master's thesis

• Title (english): Effect of perinatal treatment with serotonin agonists on immunological and hematological parameters in adult rats
• Author: Valentina Lipovec
• Mentor: Dubravka Hranilović (mentor)
• Committee member: Dubravka Hranilović (predsjednik povjerenstva)
• Committee member: Mirjana Kalafatić (član povjerenstva)
• Committee member: Mirta Tkalec (član povjerenstva)
• Committee member: Vesna Benković (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2012-12-14, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Serotonin (5HT) je biogeni amin prisutan u središnjem živčanom sustavu i perifernim tkivima, uključujući i sastavnice imunološkog sustava. U doba embrionalnog i fetalnog razvoja 5HT regulira razvoj mozga, a kasnije djeluje kao signalna molekula koja posreduje brojne fiziološke funkcije. U ovom radu istraživan je utjecaj promijenjene homeostaze serotonina, izazvane perinatalnom primjenom agonista 5-hidroksitriptofana (5HTP) ili tranilcipromina (TCP), na imunološke i hematološke parametre (broj stanica u limfnim čvorovima, slezeni i krvi, diferencijalnu krvnu sliku te razinu citokina u serumu) u zdravih odraslih štakora. Perinatalna izloženost povišenim koncentracijama serotonina značajno je smanjila broj trombocita u obje eksperimentalne skupine, značajno je povećala ukupan broj leukocita, kao i broj limfocita i neutrofila, u skupini tretiranoj TCP-om te je indikativno utjecala na razinu citokina IL-1a i RANTES u serumu tretiranih životinja. Rezultati upućuju na moguću ulogu serotonina u hematopoezi i imunološkom odgovoru, koja će biti detaljnije istražena nakon poticanja imunološkog odgovora.

Abstract (english)

Serotonin (5HT) is a biogenic amine present in the central nervous system and peripheral tissues, including components of the immune system. During embryonic and fetal development 5HT regulates neurogenesis, while later it acts as a signaling molecule mediating various physiological functions. In this thesis, the influence of the disturbed 5HT homeostasis induced by perinatal treatment with agonists 5-hydroxytryptophan (5HTP) or tranylcypromine (TCP), on the immunological and hematological parameters (number of cells in the lymph nodes, spleen and blood, differential blood count and serum cytokine levels) has been studied in healthy adult rats. Perinatal exposure to the increased 5HT levels has significantly decreased number of platelets in both experimental groups, significantly increased total number of leukocytes, as well as numbers of lymphocytes and neutrophils, in TCP-treated rats, and indicatively influenced levels of cytokines Il-1a and RANTES in serum of the treated animals. The results indicate possible role of serotonin in hematopoiesis and immune response, which will be further explored after inducing the immune response.

• Keywords: 5-hidroksitriptofan; tranilcipromin; homeostaza; citokini; krvne stanice
• Keywords (english): 5-hydroxytryptophan; tranylcipromine; homeostasis; cytokines; blood cells
• Language: croatian
• URN:NBN: urn:nbn:hr:217:118459
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-02 09:22:34