Utjecaj hranjive podloge na specijalizirane metabolite dviju sorti vinove loze (Vitis vinifera L.) u uvjetima in vitro

master's thesis

• Title (english): Effect of nutrient medium on the specialized metabolites of two grepevines (Vitis vinifera L.) cultivars in vitro
• Author: Iva Spudić
• Mentor: Darko Preiner (mentor)
• Mentor: Zlatko Liber (komentor)
• Committee member: Zlatko Liber (predsjednik povjerenstva)
• Committee member: Vesna Petrović Peroković (član povjerenstva)
• Committee member: Mirela Sertić Perić (član povjerenstva)
• Committee member: Draginja Mrvoš-Sermek (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2022-09-26, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Cilj ovoga rada bio je uzgojiti dvije sorte vinove loze 'Portan' i 'Graševinu' u uvjetima in vitro na
dvije različite hranjive podloge, uobičajenoj podlozi koja sadrži Murashige i Skoog medij te
podlozi koja osim navedenog medija sadrži i usporivač rasta, alar B9. Biljke uzgojene u uvjetima
usporenoga rasta bile su manje u vidu kraćih stabljika i manjih dimenzija listova u odnosu na biljke
uzgojene na uobičajenoj podlozi. Istražen je utjecaj različitih podloga na sastav i sadržaj
pojedinačnih polifenolnih te hlapljivih organskih spojeva u listovima navedenih sorti. Veće
vrijednosti masenih udjela gotovo svih analiziranih polifenolnih spojeva dobivene su u uzorcima
listova biljaka uzgojenih na uobičajenoj podlozi, dok su gotovo svi pojedinačni hlapljivi spojevi
bez obzira u koju kemijsku skupinu pripadaju (terpenoide, kiseline, aldehide, ketone i alkohole)
imali veće apsolutne površine pikova u uvjetima usporenog rasta. Dobiveni rezultati potvrđuju da
uporaba hranjive podloge za usporeni rast nema negativan utjecaj na biljku čime je utvrđena
prikladnost njene uporabe za potrebe čuvanja genetskog materijala vinove loze u uvjetima in vitro,
a čime se smanjuje učestalost subkultivacije biljaka.

Abstract (english)

The aim of this work was to grow two grape varieties 'Portan' and 'Graševina' under in vitro
conditions on two different nutrient media, the usual medium containing Murashige and Skoog
medium and the medium containing, in addition to the mentioned medium, a growth retarder, alar
B9. Plants grown under conditions of slow growth were smaller in terms of shorter stems and
smaller leaf sizes compared to plants grown on the usual substrate. The influence of different
nutrient media on the composition and content of individual polyphenolic and volatile organic
compounds in the leaves of the mentioned varieties was tested. Higher values of content of almost
all analyzed polyphenolic compounds were obtained in leaf samples of plants grown on the usual
substrate, while almost all individual volatile compounds, regardless of which chemical group they
belong to (terpenoids, acids, aldehydes, ketones, and alcohols), had greater responses in the slow
growth conditions. The obtained results confirm that the use of nutrient medium for slow growth
does not have a negative effect on the plant, which established the suitability of its use for the
purposes of preserving the genetic material of the grapevine in vitro, which reduces the frequency
of subcultivation of plants.

• Keywords: 'Portan'; 'Graševina'; polifenolni spojevi; hlapljivi spojevi
• Keywords (english): 'Graševina'; 'Portan'; polifpehnolic compounds; volatile organic compounds
• Language: croatian
• URN:NBN: urn:nbn:hr:217:935990
• Study programme: Title: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2024-10-01
• Created on: 2022-10-25 11:03:15