Utjecaj polimorfizma u genima za biosintezu gangliozida i gangliozidnoga sastava membrana limfocita T na razvoj karcinoma vrata maternice

doctoral thesis

• Title (english): Role of ganglioside biosynthesis genetic polymorphism and gangliosides expression in T lymphocytes membranes in cervical cancer development
• Author: Damir Danolić
• Mentor: Marija Heffer (mentor)
• Mentor: Mario Puljiz (sumentor); MBZ: 337403
• Committee member: Maja Matulić (predsjednik povjerenstva)
• Committee member: Svjetlana Kalanj-Bognar (član povjerenstva); MBZ: 202561
• Committee member: Slavko Orešković (član povjerenstva); MBZ: 205340
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2016, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology
• Universal decimal classification (UDC): 576 - Cellular and subcellular biology. Cytology

Abstract

Katedra za medicinsku biologiju i genetiku, Medicinski fakultet u Osijeku Karcinom vrata maternice najčešći je karcinom ženskog spolnog sustava u svijetu. Tijekom imunološkog odgovora na infekciju humanim papiloma virusom dolazi do premještanja T-staničnog receptora unutar lipidnih splavi limfocita T, u kojima gangliozidi imaju aktivnu ulogu u oblikovanju, stabilizaciji i biološkoj funkciji. Svrha istraživanja je utvrditi postoji li povezanost pojedinih polimorfizama u genima za biosintezu gangliozida i gangliozidnoga sastava membrana limfocita T s pojavom karcinoma vrata maternice. Genotipizacija polimorfizama gena za biosintezu gangliozida učinjena je primjenom metodologije Taqman. Sve ispitanice bile su homozigoti i imale su divlji tip alela kod svih ispitivanih gena (CC za gen B4GALT5, AA za gen ST3GAL5, AA za gen ST8SIA1 i CC za gen B4GALNT1). Analiza gangliozidnoga sastava staničnih membrana limfocita T provedena je korištenjem dvostrukog označavanja protutijelima na gangliozid GM1 i na antigene pojedinih loza limfocita. Nismo našli razliku u gangliozidnom sastavu membrana limfocita T između istraživanih skupina.

Abstract (english)

Department of Medical Biology and Genetics, Faculty of Medicine, University of Osijek Cervical cancer is the most common gynaecological cancer in women. Cell mediated immunity plays a significiant role in progression or regression of neoplastic cervical lesions caused by human papilloma virus infection. Engagement of antigen-specific T cell receptors is a prerequisite for T cell activation. The initial events of T cell activation involves movement of the T cell receptor into specialized microdomains known as lipid rafts. Gangliosides play an active role in the formation, stabilization and biological functions of lipid rafts. This study aims to determine whether polymorphisms in the genes involved in the biosynthesis of gangliosides and gangliosides expression in T cell membrane represent risk factor for cervical cancer. Taqman methods for single nucleotide polymorphism genotyping was used. All subjects carried the homozygous wild-type genotypes for all analyzed genes (CC for gene B4GALT5, AA for gene ST3GAL5, AA for gene ST8SIA1 and CC for gene B4GALNT1). Gangliosides expression in T lymphocytes membranes was analyzed using double labelling antibodies on ganglioside GM1. We have not found difference in gangliosides expression in T lymphocytes membranes between the two groups.

• Keywords: karcinom vrata maternice; stanična imunost; lipidne splavi; gangliozidi; polimorfizam
• Keywords (english): cervical cancer; lipid rafts; cell-mediated immunity; gangliosides; polymorphism
• Language: croatian
• URN:NBN: urn:nbn:hr:217:265561
• Study programme: Title: Biology; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Closed access
• Created on: 2017-05-12 09:46:38