prikaz prve stranice dokumenta Naegleria’s mitotic spindles are built from unique tubulins and highlight core spindle features
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Scientific paper - Original scientific paper
Naegleria’s mitotic spindles are built from unique tubulins and highlight core spindle features
Current Biology, 32 (2022), 6; 1247-1261.e6. https://doi.org/10.1016/j.cub.2022.01.034
Velle, Katrina B.; Kennard, Andrew S.; Trupinić, Monika; Ivec, Arian; Swafford, Andrew J.M.; Nolton, Emily; Rice, Luke M.; Tolić, Iva M.; Fritz-Laylin, Lillian K.; Wadsworth, Patricia

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Velle, K. B., Kennard, A. S., Trupinić, M., Ivec, A., Swafford, A. J., Nolton, E. ... Wadsworth, P. (2022). Naegleria’s mitotic spindles are built from unique tubulins and highlight core spindle features. Current Biology, 32. (6), 1247-1261.e6. doi: 10.1016/j.cub.2022.01.034

Velle, Katrina B., et al. "Naegleria’s mitotic spindles are built from unique tubulins and highlight core spindle features." Current Biology, vol. 32, no. 6, 2022, pp. 1247-1261.e6. https://doi.org/10.1016/j.cub.2022.01.034

Velle, Katrina B., Andrew S. Kennard, Monika Trupinić, Arian Ivec, Andrew J.M. Swafford, Emily Nolton, Luke M. Rice, Iva M. Tolić, Lillian K. Fritz Laylin and Patricia Wadsworth. "Naegleria’s mitotic spindles are built from unique tubulins and highlight core spindle features." Current Biology 32, no. 6 (2022): 1247-1261.e6. https://doi.org/10.1016/j.cub.2022.01.034

Velle, K. B., et al. (2022) 'Naegleria’s mitotic spindles are built from unique tubulins and highlight core spindle features', Current Biology, 32(6), pp. 1247-1261.e6. doi: 10.1016/j.cub.2022.01.034

Velle KB, Kennard AS, Trupinić M, Ivec A, Swafford AJ, Nolton E, and sur.. Naegleria’s mitotic spindles are built from unique tubulins and highlight core spindle features. Current Biology [Internet]. 2022 March [cited 2024 September 11];32(6):1247-1261.e6. doi: 10.1016/j.cub.2022.01.034

K. B. Velle, et al., "Naegleria’s mitotic spindles are built from unique tubulins and highlight core spindle features", Current Biology, vol. 32, no. 6, pp. 1247-1261.e6, March 2022. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:217:530398. [Accessed: 11 September 2024]

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Metadata
Title (english)Naegleria’s mitotic spindles are built from unique tubulins and highlight core spindle features
AuthorKatrina B. Velle
AuthorAndrew S. Kennard
AuthorMonika Trupinić
AuthorArian Ivec
AuthorAndrew J.M. Swafford
AuthorEmily Nolton
AuthorLuke M. Rice
AuthorIva M. Tolić
AuthorLillian K. Fritz-Laylin
AuthorPatricia Wadsworth
Author's institutionUniversity of Zagreb
Faculty of Science
(Department of Physics)
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Physics
Abstract (english)
Naegleria gruberi is a unicellular eukaryote whose evolutionary distance from animals and fungi has made it useful for developing hypotheses about the last common eukaryotic ancestor. Naegleria amoebae lack a cytoplasmic microtubule cytoskeleton and assemble microtubules only during mitosis and thus represent a unique system for studying the evolution and functional specificity of mitotic tubulins and the spindles they assemble. Previous studies show that Naegleria amoebae express a divergent α-tubulin during mitosis, and we now show that Naegleria amoebae express a second mitotic α- and two mitotic β-tubulins. The mitotic tubulins are evolutionarily divergent relative to typical α- and β-tubulins and contain residues that suggest distinct microtubule properties. These distinct residues are conserved in mitotic tubulin homologs of the “brain-eating amoeba” Naegleria fowleri, making them potential drug targets. Using quantitative light microscopy, we find that Naegleria’s mitotic spindle is a distinctive barrel-like structure built from a ring of microtubule bundles. Similar to those of other species, Naegleria’s spindle is twisted, and its length increases during mitosis, suggesting that these aspects of mitosis are ancestral features. Because bundle numbers change during metaphase, we hypothesize that the initial bundles represent kinetochore fibers and secondary bundles function as bridging fibers.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleCurrent Biology
Numberingvol. 32, no. 6, pp. 1247-1261.e6
p-ISSN0960-9822
DOIhttps://doi.org/10.1016/j.cub.2022.01.034
URN:NBNurn:nbn:hr:217:530398
Publication2022-03
ProjectNumber: IP-2019-04-5967
Title (croatian): Ravnoteža sila i momenta sila u diobenom vretenu
Title (english): Balance of forces and torques in the mitotic spindle
Acronym: SpindleForceBalance
Leader: Nenad Pavin
Jurisdiction: Croatia
Funding stream: Research Projects
ProjectNumber: KK.01.1.1.01.0004
Title (croatian): Provedba vrhunskih istraživanja u sklopu Znanstvenog centra izvrsnosti za kvantne i kompleksne sustave te reprezentacije Liejevih algebri
Title (english): Implementation of top research within the Scientific Center of Excellence for Quantum and Complex Systems, and Representations of Lie Algebras
Acronym: QuantiXLie
Leader: Hrvoje Buljan
Jurisdiction: eu
Funding stream: European Regional Development Fund
ProjectNumber: KK.01.1.1.04.0057
Title (croatian): Inovativni protokoli mikroskopije za interdisciplinarna istraživanja u biomedicini
Title (english): Inovative microscopy protocols for interdisciplinary research in biomedicine
Acronym: IPSted
Leader: Iva Marija Tolić
Jurisdiction: eu
Funding stream: European Regional Development Fund
ProjectNumber: 855158
Title (croatian): Molekularno podrijetlo aneuploidija u zdravim i bolesnim ljudskim tkivima
Title (english): Molecular origins of aneuploidies in healthy and diseased human tissues
Acronym: ANEUPLOIDY
Leader: Iva Marija Tolić
Jurisdiction: eu
Document URLhttps://linkinghub.elsevier.com/retrieve/pii/S0960982222000458
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Public note© 2022 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). Received: June 29, 2021. Received in revised form: November 2, 2021. Accepted: January 12, 2022. Published: February 8, 2022.
Created on2024-08-22 20:05:05