Utjecaj oksidativnoga stresa na sintezu hormona rasta u neuronskom tkivu

doctoral thesis

• Title (english): Oxidative stress impact on growth hormone creation in neuronal tissue
• Author: Borna Šarić
• Mentor: Branimir Cerovski (mentor); MBZ: 144926
• Committee member: Vesna Benković (član povjerenstva)
• Committee member: Nenad Vukojević (član povjerenstva); MBZ: 224951
• Committee member: Tihomir Balog (član povjerenstva); MBZ: 218820
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2015, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology
• Universal decimal classification (UDC): 577 - Biochemistry. Molecular biology. Biophysics

Abstract

Ova studija je osmišljena kako bi procijenili utjecaj oksidativnog stresa na extrapituitarnu sekreciju hormona rasta (GH) u oku, kao i analizirali međuovisnost razina produkcije očnog i serumskog GH u normalnim i uvjetima hipoksije. Ispitanici koji su predstavljali model oksidativnog stresa su 32 pacijenata s razvijenom proliferativnom dijabetičkom retinopatijom (PDR) i bili su liječeni kirurški pars plana vitrektomija (PPV). 49 pacijenata, ne-dijabetičara u kontrolnoj skupini (K) su izabrani između onih koji nisu patili od metaboličke bolesti, a ipak su morali biti operirani zbog drugih očnih bolesti. Uzorci vitreusa uzimani su tijekom PPV kirurškog zahvata, a istovremeno je uzeta i krv iz kubitalne vene. Mjerenja GH vrijednosti u serumu i vitreusu provedena su testom elektrokemijske luminiscencije (ECLIA). Procjena oksidativnog stresa izvedena je mjerenjem produkata uznapredovale oksidacije proteina (AOPP) i lipidne peroksidacije (LPO) pomoću testa enzimski vezane imunoabsorpcije (ELISA) u serumu i vitreusu. Serumske vrijednosti AOPP su bile značajno više nego vitrealne u obje skupine (p <0,001 za svaku skupinu), a za LPO samo u PDR skupini (p <0,001). Postoji značajna pozitivna korelacija između serumskih i vitrealnih vrijednosti LPO u skupini dijabetičara (r = 0,909, p <0,001).Vrijednosti GH u obije skupine značajno su veće u serumu, nego vitreusu (p <0,001). Usporedbom vrijednosti GH u serumu između kontrolne i PDR skupine, značajno više vrijednosti su pronađene kod dijabetičara (p = 0,012). Vitrealne vrijednosti GH izmjerene u obje skupine su slične, nešto više u dijabetičara. Rezultati našeg istraživanja su potvrdili postojanje sekrecije GH u oku i otkrili da je njegova produkcija u oku samostalna i neovisna o GH hipofize, a također je neovisna o utjecaju oksidativnog stresa.

Abstract (english)

This study was designed to evaluate influence of oxidative stress environment on extrapituitary growth hormone (GH) secretion in the eye as well as to analize interdependence between eye GH levels and serum GH levels in normal and hypoxic conditions. 32 patients with developed proliferative diabetic retinopathy (PDR) represented oxidative stress model and were treated surgically by pars plana vitrectomy (PPV). 49 patients, non-diabetics in control group were selected from those who have not suffered from metabolic diseases and yet they had to be operated due to other ocular disorders. Vitreous samples were taken during PPV surgical procedure and simultaneously blood from the cubital vein was collected. Measurements of GH values in serum and vitreous samples were conducted by electrochemical luminescence assay (ECLIA). Evaluation of oxidathive stress was carried out using enzyme-linked immunosorbent assay (ELISA) of Advanced Oxidation Protein Products (AOPP) and Lipide Hydroperoxide (LPO) in serum and vitreous as well. Serum values of AOPP were significantly higher than vitreal in both groups (p<0,001 for each group), and for LPO only in PDR group (p<0,001). Significant positive correlation between serum and vitreous LPO values in diabetic group (r=0,909; p<0,001) was observed. GH values in both groups were significantly higher in serum, than vitreous (p<0,001 for each group) and comparing GH serum values between control and PDR group, significantly higher amounts were found in diabetics (p=0,012). Vitreous GH values measured in both groups were similar, slightly higher in diabetics. Results of our study have confirmed existence of GH secresion in the eye and disclosed that GH production in eye is autonomous and independent to pituitary GH and is also independent to oxidative stress influence.

• Keywords: oksidativni stres; hormon rasta
• Keywords (english): oxidative stress; growth hormone
• Language: croatian
• URN:NBN: urn:nbn:hr:217:924932
• Study programme: Title: Biology; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-05-17 10:49:50