Uloga gena KIR u transplantaciji krvotvornih matičnih stanica

doctoral thesis

• Title (english): The role of KIR genes in hematopoietic stem cell transplantation
• Author: Marija Burek Kamenarić
• Mentor: Renata Žunec (mentor); MBZ: 245890
• Committee member: Zorana Grubić (član povjerenstva)
• Committee member: Vesna Benković (član povjerenstva)
• Committee member: Alenka Gagro (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2014, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology
• Universal decimal classification (UDC): 575 - General genetics. General cytogenetics

Abstract

Receptori prirodnoubilačkih stanica slični imunoglobulinu (engl. killer cell immunoglobulin-like receptor, KIR) su obitelj inhibicijskih i aktivacijskih receptora ispoljenih na prirodnoubilačkim stanicama (NK), a osnovna funkcija im je regulacija aktivnosti stanica NK. Receptori KIR imaju utjecaj na tijek i ishod transplantacije te su odgovorni za stvaranje aloreaktivnih stanica NK prilikom transplantacije krvotvornih matičnih stanica (TKMS). Kako bi se istražila uloga receptora KIR u imunologiji transplantacijske reakcije, provedeno je temeljno istraživanje polimorfizama i učestalosti gena, genotipova i haplotipova KIR na reprezentativnom uzorku hrvatske populacije (N=125). Retrogradnom analizom 111 bolesnika liječenih TKMS od srodnog i nesrodnog davatelja, istražen je utjecaj gena KIR na čimbenike ishoda TKMS: preživljavanje, GvHD i postizanje punog kimerizma. Dobiveni rezultati ukazuju da ligandi HLA-C skupine C1 i C2 primatelja kao i genotip KIR primatelja i davatelja imaju pozitivan učinak na preživljavanje i pojavu GvHD-a, dok na postizanje punog kimerizma nemaju nikakav učinak.

Abstract (english)

Killer cell immunoglobulin-like receptors (KIR) are family of inhibitory and activatory receptors expressed on natural killer cells (NK) with the basic role of regulation the NK cell activity. KIRs have influence on the hematopoietic stem cell transplantation (HSCT) outcome and are responsible for generating alloreactive NK cells. To explore the KIR gene role in HSCT, we performed the basic investigation of gene polymorphisms and KIR gene, haplotype and genotype frequencies in the group of healthy unrelated individuals (N=125) that represents the Croatian population and serve as a control group in the further studies. The role of KIR genes in HSCT in terms of disease free survival, the intensity of GvHD and chimerism was evaluated by retrograde analyses of 111 patients and their related and unrelated donors. The results showed that the HLA-C ligands and the donor-recipient KIR genotypes have major influence on the survival and GvHD effect, while there is no effect on achieving full chimerism after HSCT.

• Keywords: geni KIR; stanice NK; hrvatska populacija; transplantacija krvotvornih matičnih stanica; GvHD; preživljavanje; kimerizam
• Keywords (english): KIR genes; NK cells; Croatian population; Haematopoietic stem cell transplantation; GvHD; survival; chimerism
• Language: croatian
• URN:NBN: urn:nbn:hr:217:618081
• Study programme: Title: Biology; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-05-17 12:32:12