Duljina telomera i imunoreaktivnost u posttraumatskom stresnom poremećaju

doctoral thesis

• Title (english): Telomere length and immunoreactivity in posttraumatic stress disorder
• Author: Mladen Jergović
• Mentor: Krešo Bendelja (mentor)
• Mentor: Anđelko Vidović (sumentor)
• Committee member: Ivica Rubelj (član povjerenstva)
• Committee member: Nada Oršolić (član povjerenstva)
• Committee member: Neda Slade (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2014, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology
• Universal decimal classification (UDC): 577 - Biochemistry. Molecular biology. Biophysics

Abstract

Sve je više dokaza da kronični stres ubrzava skraćivanje telomera u leukocitima i mononuklearnim stanicama iz periferne krvi. Funkcionalne posljedice skraćivanja telomera nisu razjašnjene. U ovom istraživanju mjerena je duljina telomera i aktivnost telomeraze u mononuklearnim stanicama iz periferne krvi 62 osobe: (pacijenti oboljeli od posttraumatskog stresnog poremećaja (PTSP) (N = 30); zdrave kontrole (N = 17), starije osobe (N = 15)). Paralelno, izmjerena je proliferacija aktiviranih T-limfocita i njihova proizvodnja citokina interferona (IFN)-γ, interleukina (IL)-2, IL-4; proizvodnja faktora nekroze tumora (TNF)-α i IL-6 od strane monocita te izraženost receptora programmed death 1 (PD-1) i njegovog liganda PD-L1 na aktiviranim T-limfocitima. Ovo istraživanje pridonosi rastućem broju dokaza da psihološka trauma i kronični stres doprinose skraćivanju telomera. Iako manje izraženi kod pacijenata oboljelih od PTSP-a nego kod starijih osoba, smanjeni proliferativni odgovori T-limfocita i kraća duljina telomera mogli bi biti znakovi rane imunosenescencije. Zajedno sa smanjenom proizvodnjom Th1-citokina, ove promjene imunološkog sustava mogle bi doprinijeti zdravstvenim rizicima povezanim s PTSP-om.

Abstract (english)

There is increasing evidence that chronic stress accelerates telomere erosion in leukocytes/ peripheral blood mononuclear cells (PBMCs). However, functional changes associated with telomere shortening are poorly understood. We measured relative telomere length and basal telomerase activity in PBMCs of 62 individuals (patients with posttraumatic stress disorder (PTSD) (N=30); age-matched healthy controls (N=17), elderly volunteers (N=15)). In parallel, we have assessed proliferation of activated T cells, interferon (IFN)-γ, interleukin (IL)-2, IL-4, tumor necrosis factor (TNF)-α and IL-6 cytokine production and expression of programmed death 1 (PD-1) receptor and its ligand PD-L1 on activated T cells. This study adds to the accumulating evidence that psychological trauma and chronic stress are associated with accelerated telomere attrition. Although much less pronounced in PTSD patients than in elderly persons, reduced proliferative responses of T cells accompanied by shorter telomeres might be a sign of early immunosenescence. Together with reduced production of Th1 cytokines, observed immune changes may contribute to health risks associated with PTSD.

• Keywords: telomere; posttraumatski stresni poremećaj; imunost; imunosenescencija
• Keywords (english): telomeres; posttraumatic stress disorder; immunity; immunosenescence
• Language: croatian
• URN:NBN: urn:nbn:hr:217:427089
• Study programme: Title: Biology; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-05-18 09:27:36