Raznolikost gena glavnoga sustava tkivne podudarnosti skupine II u konja prirodno inficiranih patogenim bakterijama iz roda Leptospira

doctoral thesis

• Title (english): Genetic diversity of major histocompatibility complex class II in horses naturally infected with pathogenic bacteria of the genus Leptospira
• Author: Vesna Mojčec Perko
• Mentor: Nenad Turk (mentor); MBZ: 205224
• Mentor: Ana Galov (sumentor)
• Committee member: Jasna Hrenović (član povjerenstva)
• Committee member: Haidi Arbanasić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2014, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology
• Universal decimal classification (UDC): 575 - General genetics. General cytogenetics

Abstract

Leptospiroza je akutna septikemijska zarazna bolest brojnih vrsta domaćih i divljih životinja, ali i čovjeka, uzrokovana patogenim bakterijama iz roda Leptospira. U konja na području Republike Hrvatske utvrđena je visoka seroprevalencija leptospiroze. Izrazito važnu ulogu u prepoznavanju patogenih mikroorganizama i pokretanju imunosnog odgovora domaćina ima glavni sustav tkivne podudarnosti skupine II (MHC II). U ovom istraživanju analiziran je glavni nositelj genske raznolikosti MHC II regije - egzon 2, DRA i DRB lokusa u 200 konja, 100 inficiranih leptospirama i 100 neinficiranih. Pronađeno je pet DRA i 14 DRB alela, od čega je šest DRB alela utvrđeno po prvi puta. Svaki alel daje jedinstveni aminokiselinski slijed. Utvrđen je 41 DRA/DRB haplotip. Visoka raznolikost alela i postojanje transspecijskog polimorfizma pokazatelji su djelovanja ravnotežne selekcije na lokusima. Statističkim analizama su utvrđene naznake pravila grupiranja jedinki prema haplotipu i serovaru leptospira na koji jedinka pokazuje serološki odgovor, no zbog malog broja jedinki koje pripadaju pojedinoj kategoriji nije moguće pouzdano utvrditi općenita pravila koja bi vrijedila za cjelokupnu populaciju konja.

Abstract (english)

Leptospirosis is a zoonotic disease of worldwide distribution caused by pathogenic bacteria of the genus Leptospira. High seroprevalence of leptospirosis was determined in horse population of Croatia. The major histocompatibility complex class II (MHC II) has a crucial role for patogen presentation to cells of the immune system and initiation of the immune response. The main carrier of genetic variability of MHC II region is exon 2. The genetic diversity and evolutionary history of the exon 2 DRA and DRB loci were analysed in 200 horses, 100 leptospira infected and 100 uninfected. Five DRA and 14 DRB alleles were detected, while six DRB alleles were new. Each allele results in a single amino acid sequence. 41 haplotypes were found. Highly diverged allele sequences and occurrence of trans-species polymorphism provide evidence for action of balancing selection on both loci. The statistical analyzes showed some indications of the rules of grouping individuals according to haplotype and leptospira serovar in which an individual shows serologic response, but due to the small number of individuals in a particular category is not possible to apply certain rules to the entire population of horses.

• Keywords: Equus caballus; leptospiroza; MHC; DRA; DRB
• Keywords (english): Equus caballus; leptospirosis; MHC; DRA; DRB
• Language: croatian
• URN:NBN: urn:nbn:hr:217:828838
• Study programme: Title: Biology; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-05-18 10:25:19