Utjecaj poremećene središnje homeostaze monoamina na metabolizam lipida, aktivnost monoaminooksidaze tipa B i koncentraciju serotonina u trombocitima

doctoral thesis

• Title (english): The impact of disrupted monoamine homeostasis on lipid metabolism, activity of monoamine oxidase type b and serotonin concentration in platelets
• Author: Antonia Puljić
• Mentor: Darko Marčinko (mentor); MBZ: 328484
• Mentor: Nela Pivac (sumentor)
• Committee member: Dorotea Muck-Šeler (član povjerenstva)
• Committee member: Vlado Jukić (član povjerenstva); MBZ: 173210
• Committee member: Dubravka Hranilović (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2014, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology
• Universal decimal classification (UDC): 577 - Biochemistry. Molecular biology. Biophysics

Abstract

Poremećaj središnje homeostaze monoamina, kao podloga različitih psihijatrijskih poremećaja, pa tako i poremećaja ličnosti i suicidalnog ponašanja, utječe na metabolizam lipida i aktivnost monoaminooksidaze tipa B (MAO-B) i koncentraciju serotonina (5-HT) u trombocitima. Cilj istraživanja je bio odrediti utjecaj promijenjene homeostaze središnjih monoamina na metabolizam lipida te aktivnost MAO-B i koncentraciju 5-HT u trombocitima bolesnica s poremećajem ličnosti sa i bez suicidalnog ponašanja te ih usporediti s vrijednostima izmjerenim u zdravim kontrolnim ispitanicama. Metode: Lipidne frakcije i trombocitni pokazatelji određeni su u uzorcima krvi ispitanica laboratorijskim i spektrofluorimetrijskim metodama. Rezultati su obrađeni pomoću analiza varijance, Tukeyovog testa, Student t-testa, regresijske analize i Spearmanovog koeficijenta korelacije. Rezultati: Koncentracija ukupnog kolesterola značajno je snižena u suicidalnih bolesnica u odnosu na kontrolne ispitanice, koncentracija trombocitnog 5-HT i LDL-a značajno je snižena u suicidalnih i nesuicidalnih bolesnica u odnosu na kontrolnu skupinu, a koncentracija triglicerida značajno je povišena kod nesuicidalnih bolesnica u odnosu na kontrolnu skupinu. Trombocitni 5-HT bio je značajno povezan s kolesterolom i LDL-kolesterolom. Zaključak: Koncentracije trombocitnog 5-HT i LDL-kolesterola mogle bi poslužiti kao indikatori poremećaja ličnosti, dok je koncentracija ukupnog kolesterola jedini periferni pokazatelj koji razlikuje suicidalne i nesuicidalne ispitanice te bi mogao poslužiti kao potencijalni marker suicidalnosti. Rezultati upućuju da je smanjena periferna monoaminska funkcija, povezana s kolesterolom, karakteristična značajka poremećaja ličnosti.

Abstract (english)

The disruption of central monoamine homeostasis, as the basis of personality disorders and suicidal behaviour, affects lipid metabolism and monoamine oxidase type B (MAO-B) activity and serotonin (5-HT) concentration in platelets. Aim was to determine the influence of this altered homeostasis on lipid and platelet markers in female personality disorder patients with and without suicidal behaviour and to compare these markers with values in healthy control women. Methods: Lipid fractions and platelet markers were determined in blood samples of subjects with standard laboratory and spectrofluorimetric methods. Results were evaluated with analysis of variance, Tukey test, Student t-test, regression analysis and Spearman coefficient of correlation. Results: Serum total cholesterol was significantly lower in suicidal patients compared to control subjects, concentration of platelet 5-HT and LDL-cholesterol was significantly lower in suicidal and nonsuicidal patients compared to controls, and concentration of triglycerides was significantly higher in nonsuicidal patients compared to controls. Platelet 5-HT was significantly positively correlated with cholesterol and LDL-cholesterol. Conclusion: The concentration of platelet 5-HT and LDL cholesterol could serve as indicators of personality disorders, while serum total cholesterol, which is the only peripheral indicator that distinguishes suicidal and nonsuicidal patients, could serve as a potential indicator of suicididality. The results suggest that the reduced peripheral monoamine function, associated with cholesterol, is a characteristic feature of personality disorders.

• Keywords: središnji monoamini; poremećaj homeostaze; trigliceridi; kolesterol; LDL-kolesterol; HDL-kolesterol; trombocitni serotonin; trombocitna monoaminooksidaza; suicidalnost; poremećaj ličnosti
• Keywords (english): central monoamines; disrupted homeostasis; triglycerides; cholesterol; LDL- cholesterol; HDL- cholesterol; platelet serotonin; platelet monoamine oxidase; suicidality; personality disorder
• Language: croatian
• URN:NBN: urn:nbn:hr:217:644699
• Study programme: Title: Biology; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Closed access
• Created on: 2017-05-18 11:15:13