Strukturna analiza N-glikoma krvne plazme dugoživućeg golokrtičastoga štakora (Heterocephalus glaber Rüppell, 1842)

doctoral thesis

• Title (english): Structural analysis of blood plasma N-glycome from a long-lived naked mole-rat (Heterocephalus glaber Rüppell, 1842)
• Author: Siniša Habazin
• Mentor: Mislav Novokmet (mentor)
• Committee member: Toma Keser (predsjednik povjerenstva)
• Committee member: Biljana Balen (član povjerenstva)
• Committee member: Göran Klobučar (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2025-02-24, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology
• Universal decimal classification (UDC): 57 - Biological sciences. Physical anthropology. Biodiversity

Abstract

Golokrtičasti štakor (GŠ) (Heterocephalus glaber Rüppell, 1842) je eusocijalni fosorijalni glodavac čiji je prirodni areal Istočna Afrika, a zbog životnog vijeka koji prelazi 30 godina je atraktivan modelni organizam u biologiji starenja. Molekularni mehanizmi otpornosti na kancerogenezu, neurodegeneraciju, hipoksiju i neke vrste boli povezani su sa održavanjem proteostaze u čemu sudjeluju i posttranslacijske modifikacije proteina. Dinamika i raznolikost glikozilacije proteina u GŠ do sada nije... More istraživana. U ovom je radu pomoću tekućinske kromatografije i spektrometrije masa provedena strukturna analiza N-glikoma krvne plazme GŠ. Određene su strukture i udjeli 160 različitih N-glikana među kojima se ističe strukturni motiv 4-O-acetilacije sijalinskih kiselina. Glikoproteomskom analizom otkriveno je da takvi sijaloglikani potječu s inhibitora proteaza u krvnoj plazmi te je pretpostavljen njihov zaštitni i stabilizirajući utjecaj na proteine. Less

Abstract (english)

The naked mole-rat (NMR) (Heterocephalus glaber Rüppell, 1842) is an eusocial fossorial rodent native to East Africa. Its exceptionally long lifespan of over 30 years makes it a popular non-conventional model organism in the biology of ageing and
longevity. Molecular mechanisms underlying resistance to cancerogenesis, neurodegeneration, hypoxia and certain types of pain are related to the maintenance of proteostasis where key players are protein post-translational modifications. The dynamics... More and diversity of protein glycosylation in NMR are completely unexplored. In this study, a structural analysis of NMR blood plasma N-glycome was performed using liquid chromatography and mass spectrometry. A total od 160 different N-glycans were annotated and quantified. The core structural motif in the N-glycome of NMR was the presence of 4-O-acetylated sialic acids. Employing a glycoproteomic analysis, 4-Oacetylated sialo-N-glycans were pinpointed to plasma protease inhibitors and their protective and stabilizing effect on the proteins was inferred. Less

• Keywords: glikozilacija; proteomika; spektrometrija masa; ugljikohidrati; starenje
• Keywords (english): glycosylation; proteomics; mass spectrometry; carbohydrates; aging
• Language: croatian
• URN:NBN: urn:nbn:hr:217:564336
• Project: Number: KK.01.2.2.03.0006; Title: Centar kompetencija u molekularnoj dijagnostici; Acronym: CEKOM; Leader: Tomislav Krističević; Jurisdiction: eu; Funder: Europska unija; Funding stream: European Regional Development Fund
• Study programme: Title: Doctoral study; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti u području prirodnih znanosti (doktor/doktorica znanosti u području prirodnih znanosti)
• Type of resource: Text
• Extent: 173 str., 58 slika, 5 tablica, 236 literaturnih navoda
• File origin: Born digital
• Access conditions: Open access
• Created on: 2025-03-03 11:00:38