Utjecaj dušikovih spojeva na fotosintetske pokazatelje u lišaja Flavoparmelia caperata

master's thesis

• Title (english): The effect of nitrogen compounds on photosynthetic parameters in lichen Flavoparmelia caperata
• Author: Marina Smetiško
• Mentor: Mirta Tkalec (mentor)
• Committee member: Mirta Tkalec (predsjednik povjerenstva)
• Committee member: Ines Radanović (član povjerenstva)
• Committee member: Marina Cindrić (član povjerenstva)
• Committee member: Vesna Petrović Peroković (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2017-09-27, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Lišaji su dobri bioindikatori kvalitete zraka pa ih se u zadnje vrijeme često koristi za praćenje emisije amonijaka i dušikovih oksida. Cilj ovog istraživanja je bio odrediti osjetljivost vrste Flavoparmelia caperata (L.) Hale na povećanu količinu dušika u obliku amonijevih i nitratnih iona te utvrditi utječe li intenzitet svjetla na učinak dušika. Uzorke lišaja sam tretirala uranjanjem u 1 mol dm-3 otopinu kalijeva nitrata, 1 mol dm-3 otopinu amonijeva nitrata te 0,5 mol dm-3 otopinu amonijeva sulftata u trajanju od 30 minuta. Dio uzoraka izlagala sam jačem intenzitetu osvjetljenja (100 µE m-2 s-1), a dio slabijem intenzitetu (40 µE m-2 s-1). Utjecaj dušika analizirala sam mjerenjem pokazatelja fluorescencije klorofila a in vivo, udjela fotosintetskih pigmenata te kvocijentom feofitinizacije nakon 3., 7. i 14. dana tretmana. Tretiranje amonijevim nitratom uzrokovalo je sniženje maksimalnog prinosa fotosustava II, sniženje udjela klorofila a, klorofila b i ukupnih karotenoida te sniženje kvocijenta feofitinizacije što ukazuje na negativan učinak ovog spoja na proces fotosinteze i degradaciju klorofila. Tretiranje amonijevim sulfatom uzrokovalo je povećanje udjela karotenoida 7. dana nakon tretmana te sniženje udjela klorofila b nakon 14. dana. Tretman kalijevim nitratom uglavnom nije uzrokovao značajnije promjene. Intenzitet svjetla nije značajnije utjecao na učinak dušikovih spojeva. Na temelju rezultata može se zaključiti da je ova vrsta osjetljiva na povećanu količinu dušikovih spojeva ali onih koji sadrže amonijeve ione, osobito amonijev nitrat.

Abstract (english)

Lichens are good bioindicators of air pollution so recently they are being used for tracking emission of the ammonium and nitrogen oxide. Purpose of this research was to determine sensitivity of the species Flavoparmelila caperata (L.) Hale to higher amount of nitrogen compounds and to find if intensity of light can influence the effect of the nitrogen. Lichen samples were incubated in 1 mol dm-3 solutions of potassium nitrate or ammonium nitrate, or 0.5 mol dm-3 solution of ammonium sulfate for 30 minutes. Part of the samples was exposed to higher intensity of light (100 µE m-2 s-1), and another one to lower intensity of light (40 µE m-2 s-1). Nitrogen effects were analyzed by the chlorophyll a fluorescence, content of photosynthetic pigments and phaeophytinization quotient 3th, 7th and 14th day after the treatment. Ammonium nitrate treatment decreased maximum quantum yield, content of chlorophyll a, chlorophyll b and carotenoids and also caused lowering of the phaeophytinization quotient indicating the negative effect of this compound on the photosynthesis process and chlorophyll degradation. Ammonium sulfate treatment increased the content of the total carotenoids 7th day after treatment and decreased the content of chlorophyll b 14th day after treatment. Potassium nitrate treatment did not cause any significant changes. Intensity of light did not statistically significant influence the effect of the nitrogen compounds. Based on the results, it could be concluded that Flavoparmelia caperata is sensible to higher amount of nitrogen compounds, but the ones that contain ammonium ions, especially ammonium nitrate.

• Keywords: Flavoparmelia caperata; lišaji; dušik; pigmenti; fotosinteza
• Keywords (english): Flavoparmelia caperata; lichens; nitrogen; pigments; photosynthesis
• Language: croatian
• URN:NBN: urn:nbn:hr:217:741035
• Study programme: Title: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2018-10-23
• Created on: 2017-10-23 13:35:23