Razvoj metode tekućinske kromatografije ultravisoke djelotvornosti za analizu razgradnih produkata lijeka elvitegravira

master's thesis

• Title (english): Development of ultra performance liquid chromatographic method for the analysis of elvitegravir degradation products
• Author: Zlata Lasić
• Mentor: Nives Galić (mentor)
• Committee member: Nives Galić (predsjednik povjerenstva)
• Committee member: Ines Primožič (član povjerenstva)
• Committee member: Biserka Prugovečki (član povjerenstva)
• Committee member: Snežana Miljanić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Chemistry); Zagreb
• Defense date and country: 2018-02-21, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry

Abstract

Elvitegravir je inhibitor HIV-1 integraze koji se u kombinaciji s drugim lijekovima koristi za
lijeĉenje virusa humane imunodeficijencije (HIV). Ispitivanje ĉistoće lijeka ukljuĉuje
analitiĉke metode kojima je cilj detekcija, identifikacija, strukturna karakterizacija i
kvantitativno odreĊivanje oneĉišćenja. U ovom radu razvijena je brza i uĉinkovita metoda
tekućinske kromatografije ultravisoke djelotvornosti (UHPLC) za analizu oneĉišćenja i
razgradnih produkata lijeka elvitegravira. U svrhu optimizacije kromatografskih uvjeta
(vremenskog gradijenta, temperature kolone i pH vrijednosti) koristio se raĉunalni program
DryLab. Za analizu razgradnih produkata, nastalih forsiranom razgradnjom, koristio se vezani
sustav LC-MS/MS. Na temelju analize MS i MS/MS spektara pretpostavljene su strukture
razgradnih produkata i naĉin njihove fragmentacije.

Abstract (english)

Elvitegravir is an HIV-1 integrase inhibitor and in combination with other drugs is used to
treat a human immunodeficiency virus (HIV). The drug purity testing includes analytical
methods aimed at detection, identification, structural characterization and quantitative
determination of impurities. In this testis, a fast and efficient ultrahigh performance liquid
chromatography (UHPLC) method for the analysis of pollution and degradation products of
elvitegravir has been developed. Software DryLab has been used for optimizing of the
chromatographic conditions: time gradient, column temperature and pH value. The LC-MS /
MS coupled system has been used for analysis of the degradation products caused by forced
degradation. By analysis of the obtained MS and MS/MS spectra, the structure of the
degradation products and their fragmentation has been assumed.

• Keywords: elvitegravir; forsirana razgradnja; MS/MS; oneĉišćenja; razgradni produkti; UHPLC
• Keywords (english): elvitegravir; MS/MS; degradation products; forced degradation; impurities; UHPLC
• Language: croatian
• URN:NBN: urn:nbn:hr:217:618530
• Study programme: Title: Graduate university studies in chemistry; specializations in: Research programme; Course: Research programme; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2020-03-02
• Created on: 2018-03-06 09:11:14