Razine molekularnih biomarkera i akumulacija metala u probavnoj žlijezdi školjkaša Anodonta exulcerata Porro, 1838 iz Visovačkog jezera

master's thesis

• Title (english): Molecular biomarker levels and metal accumulation in the digestive gland of bivalve Anodonta exulcerata Porro, 1838 from Visovac Lake
• Author: Paula Dragičević
• Mentor: Dušica Ivanković (mentor)
• Mentor: Jasna Lajtner (mentor)
• Committee member: Jasna Lajtner (predsjednik povjerenstva)
• Committee member: Renata Šoštarić (član povjerenstva)
• Committee member: Inga Marijanović (član povjerenstva)
• Committee member: Sandra Hudina (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2018-02-16, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

U krškim područjima Hrvatske prirodne koncentracije metala su uglavnom niske, zbog čega su vodeni sustavi tih područja vrlo osjetljivi na povišene koncentracije metala. Visovačko jezero dio je ekosustava u zaštićenom dijelu rijeke Krke. Dosadašnja istraživanja ukazala su na povišenje razine nekih ekotoksičnih metala u sedimentima ovog jezera kao posljedicu antropogenog djelovanja, no za bolju procjenu rizika onečišćenja metalima potrebno je istražiti njihov utjecaj na organizme. Školjkaš Anodonta exulcerata korišten je kao bioindikator u ovom radu. Analiziran je sadržaj metala u jezerskoj vodi i u probavnoj žlijezdi školjkaša s plićeg i dubljeg lokaliteta. Određeni su molekularni biomarkeri izloženosti i učinaka zagađivala: metalotioneini, ukupni glutation, ukupni proteini i malondialdehid. Analiza uzoraka jezerske vode ukazala je na relativno niske razine otopljenih metala u vodi Visovačkog jezera. Uočene su značajne razlike u bioraspoloživosti pojedinih metala u plićem i dubljem dijelu Visovačkog jezera. Analiza raspodjele metala između topljive i netopljive frakcije tkiva probavne žlijezde ukazala je na specifične obrasce raspodjeljivanja pojedinih elemenata između ovih dviju frakcija pri povećanoj akumulaciji. Koncentracije metalotioneina ukazale su na značajno veću izloženost školjkaša s dubljeg lokaliteta. Koncentracija glutationa bila je značajno povišena u školjkaša s plićeg lokaliteta.

Abstract (english)

In the karst areas of Croatia natural concentrations of metals are generally low, which is why water systems in these areas are very sensitive to elevated metal concentrations. Visovac Lake is a part of the ecosystem in the protected part of the Krka River. Previous research has shown an increase in some ecotoxic metals’ levels in sediments of this lake as a result of anthropogenic activity but in order to better assess the risk of metal contamination, it is necessary to estimate their influence on the organisms. Bivalve Anodonta exulcerata was used as a bioindicator in this paper. The content of metal was analyzed in the lake water and in the digestive gland of the bivalves from the shallower and deeper site. Molecular biomarkers of exposure and effect were measured: metallothionein, total glutathione, total protein and malondialdehyde. Analysis of lake water samples showed relatively low levels of dissolved metals in Visovac Lake water. Significant differences in the bioavailability of certain metals were observed in the shallower and deeper parts of the Lake Visovac. The analysis of the distribution of metal between the soluble and insoluble fraction of digestive gland showed specific patterns of distribution of some elements between these two fractions at increased accumulation. Concentrations of metallothionein showed a significantly greater exposure of bivalves from a deeper locality. The glutathione concentration was significantly elevated in bivalves from the shallower site.

• Keywords: bioindikator; onečišćenje; metalotioneini; glutation; malondialdehid; oksidativni stres
• Keywords (english): bioindicator; pollution; metallothioneins; glutathione; malondialdehyde; oxidative stress
• Language: croatian
• URN:NBN: urn:nbn:hr:217:532115
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2020-02-16
• Created on: 2018-03-14 12:23:30