Identifikacija i kvantifikacija proteina djelomično neuređene tercijarne strukture (IDP) ekotipova uročnjaka (Arabidopsis thaliana L.) nakon izlaganja suši

master's thesis

• Title (english): Quantification and identification of intrinsically disordered proteins (IDPs) in different ecotypes of Arabidopsis thaliana L. after exposure to drought
• Author: Laura Bračun
• Mentor: Dubravko Pavoković (mentor)
• Committee member: Dubravko Pavoković (predsjednik povjerenstva)
• Committee member: Mirta Tkalec (član povjerenstva)
• Committee member: Silvija Černi (član povjerenstva)
• Committee member: Ivana Šola (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2018-02-23, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Nedostatak vode jedan je od glavnih čimbenika okolišnog stresa, a s obzirom na klimatsku regiju, on može biti dugotrajan ili kratkotrajan i nasumičan. Sesilni organizmi poput biljaka morali su razviti posebne strategije kojima prebrođuju i preživljavaju sušna razdoblja. Neke od tih strategija variraju s obzirom na genotip. U sklopu ovog diplomskog rada, suši su izložena četiri ekotipa biljke Arabidopsis thaliana, porijeklom iz četiri različite klimatske regije. Razlike u toleranciji nedostatka vode ustanovljene su na razini proteoma. Uspoređivane su aktivnosti peroksidaze kao izravan pokazatelj jačine oksidativnog stresa, relativne koncentracije Hsp70 (engl. heat-shock protein) kao indikatora generalnog stresa, i relativne koncentracije proteina djelomično neuređene strukture (IDP) koji sudjeluju u integraciji signalnih puteva te stoga imaju bitnu ulogu u mnogim biljnim procesima, uključujući one koji odgovaraju na abiotički stres. Utvrđeno je povećanje aktivnosti peroksidaze i ekspresije Hsp70 u sušnim uvjetima u usporedbi s kontrolom. Uočena je i promjena ekspresije nekih IDP u uvjetima suše s obzirom na kontrolu. Također, spektrometrijom masa identificirano je 25 IDP, a potom je razina njihove neuređenosti određena bioinformatičkim alatima. Za 21 protein potvrđeno je da posjeduju barem jednu dugu neuređenu regiju.

Abstract (english)

Water deficit is one of the crucial factors causing environmental stress and it can be, considering a particular climate region, either long-term or short-term and random. Sessile organisms like plants had to develop special strategies in order to overcome and survive periods of drought, some of which vary depending on the genotype. This thesis involves exposure to drought of four different ecotypes of Arabidopsis thaliana native to different climate regions and establishing their relative tolerance to water deficit on the level of proteome. We compared peroxidase activities as indicator of oxidative stress and relative concentrations of Hsp70 (heatshock protein) as indicator of general stress. We also determined relative concentrations of intrinsically disordered proteins (IDPs) as they are involved in integration of various signalling pathways and therefore take part in many plant processes in general, including abiotic stress responses. Compared to control, a rise was shown in both peroxidase activity and Hsp70 expression during drought conditions. Expression of some IDPs also changed after exposure to drought in comparison to control. Also, 25 IDPs were identified by mass spectrometry and their sequence structure disorder has been evaluated by bioinformatic tools. Twenty one of them were confirmed to have at least one long disordered region.

• Keywords: neuređenost proteina; Hsp70; gvajakol peroksidaza; abiotski stres
• Keywords (english): protein disorder; Hsp70; guaiacol peroxidase; abiotic stress
• Language: croatian
• URN:NBN: urn:nbn:hr:217:350906
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2020-02-23
• Created on: 2018-03-15 13:05:22