Izučavanje reakcije 5-sulfosalicilne kiseline i rutenija(III) spektrofluorimetrijskom metodom ; Timsko poučavanje kemije u srednjoj školi - fluorescencija

master's thesis

• Title (english): Investigation of reaction between ruthenium(III) and 5-sulfosalicylic acid with spectrofluorimetric method ; Team teaching in chemistry − fluorescence
• Author: Ivana Ročić
• Mentor: Vlasta Vojković (mentor)
• Mentor: Draginja Mrvoš-Sermek (mentor)
• Committee member: Draginja Mrvoš-Sermek (predsjednik povjerenstva)
• Committee member: Nives Galić (član povjerenstva)
• Committee member: Ines Radanović (član povjerenstva)
• Committee member: Jasna Lajtner (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Chemistry); Zagreb
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2016-02-29, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry

Abstract

Ovaj diplomski rad sastoji se od dvije cjeline: istraživačkog i metodičkog dijela. U prvom
dijelu proučavana je reakcija između rutenija(III) i 5-sulfosalicilne kiseline
spektrofluorimetrijskom metodom. Rutenij(III) snažno gasi fluorescenciju 5-sulfosalicilne
kiseline u neutralnim otopinama pri pH = 7,3 (kontrolirano fosfatnim puferom) uz
konstantnu ionsku jakost μ = 0,02 mol dm−3 (održavana pomoću otopine NaCl).
Fluorimetrijska mjerenja provedena su pri λem / λex = 401 / 235 nm te λem / λex = 401 / 297 nm.
Smanjenje intenziteta fluorescencije proporcionalno je koncentraciji rutenija(III) u području
od 0,2 do 12,1 μg cm−3, ako je koncentracija 5-sulfosalicilne kiseline 3✕10−5 mol dm−3,
korelacijski koeficijent iznosi 0,995.
U metodičkom dijelu rada pod naslovom Timsko poučavanje u nastavi kemije –
fluorescencija naveden je prijedlog pripreme nastavnog sata kojeg zajedno provode
nastavnici fizike, kemije i biologije, primjeren učenicima četvrtog razreda gimnazije.
Predloženi su jednostavni pokusi za koje je detaljno razrađen radni listić za učenike i
uputa za nastavnike.

Abstract (english)

This Diploma thesis consist of two parts: the research part and the methodological part. In
the research part the interaction between 5-sulfosalicylic acid (5-SSA) and ruthenium(III)
was investigated by the spectrofluorimetric method. Ruthenium(III) can effectivelly quench
the fluorescence of 5-sulfosalicilyc acid in a neutral medium pH = 7.3 (maintained by
phosphate buffer) at constant ionic strange μ = 0.02 mol dm−3 (maintained by solution of
NaCl). The fluorimetric measurements were conducted at λem / λex = 401 / 235 nm and at
λem / λex = 401 / 297 nm. The calibration graph was linear over the range 0.2-12,1 μg cm-3
of ruthenium, if the concentration of 5-sulfosalicylic acid was 3✕10−5 mol dm−3 with a
correlation coefficient of 0.995.
The methodological part of the Thesis entitled Team teaching in chemistry − fluorescence
comprises a proposal of a preparation plan of a teaching lesson appropriate to students of
the fourth grade of high school, carried out together by teachers of physics, chemistry and
biology. Simple experiments are proposed, with detailed worksheets for students and
guidance for teachers.

• Keywords: 5-sulfosalicilna kiselina; gašenje fluorescencije; rutenij (III); timski rad; fluorescencija; učenje otkrivanjem
• Keywords (english): 5-sulfosalicylic acid; fluorescence; ruthenium(III); team work; inquiry-based learning
• Language: croatian
• URN:NBN: urn:nbn:hr:217:649304
• Study programme: Title: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2018-03-01
• Created on: 2017-03-15 14:23:13