Taloženje kalcijevih oksalata u uvjetima hiperoksalurije: utjecaj aminokiselina relevantnih za patološku biomineralizaciju

doctoral thesis

• Title (english): Precipitation of calcium oxalates under conditions of hyperoxaluria: the influence of amino acids relevant for pathological biomineralization
• Author: Anamarija Stanković
• Mentor: Berislav Marković (mentor)
• Mentor: Damir Kralj (mentor)
• Committee member: Dubravka Matković-Čalogović (predsjednik povjerenstva); MBZ: 108862
• Committee member: Davor Kovačević (član povjerenstva)
• Committee member: Jasminka Kontrec (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Chemistry); Zagreb
• Defense date and country: 2018-04-25, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry
• Universal decimal classification (UDC): 54 - Chemistry. Crystallography. Mineralogy

Abstract

U ovom radu provedena su sustavna istraživanja spontanog taloženja kalcijeva oksalata pri tri različite kompleksnosti (jednostavan sustav, NaCl sustav i sustav umjetnog urina) i pri dva početna pH (pHi = 5,0 i pHi = 9,0). U svim sustavima dominantno taloži kalcijev oksalat monohidrat (COM) osim u sustavu umjetnog urina pri pHi = 9,0, ci(C2O42-) ≥ 6,0 mmol dm-3 i ci(Ca2+) ≥ 7,5 mmol dm-3 gdje taloži smjesa COM i kalcijeva oksalata dihidrata (COD). Istraženo je djelovanje sedam aminokiselina (nepolarne - Ala, Phe; polarne - Gly, Ser, Cys i nabijene - His, Asp) na spontano taloženje kalcijeva oksalata u uvjetima hiperoksalurije (ci(Ca2+) = 7,5 mmol dm-3 i ci(C2O42-) = 6,0 mmol dm-3) s ciljem rasvjetljavanja njihove uloge na taloženje hidrata kalcijeva oksalata. U sva tri promatrana sustava zabilježen je značajan utjecaj promatranih aminokiselina na morfologiju i rast kristala COM. Pri pHi = 9,0 uočene promjene su izraženije. Jedino u sustavu umjetnog urina pri pHi = 9,0 evidentirana je promjena sastava; polarne aminokiseline i nepolarna Phe inhibiraju taloženje COM.
U ovom radu je po prvi puta sintetiziran COD uz primjenu ultrazvučnog miješanja. Kristalno sjeme COD habitusa u obliku pravilnih tetragonalnih bipiramida s prizmom pripremljeno je u ultrazvučno mješanom sustavu: ci(Ca2+) = 9,00 mmol dm-3, ci(C2O42-) = 2,33 mmol dm-3 i c(citrat) = 9,00 mmol dm-3 (ci(Ca2+)/ci(C2O42-) = 3,86). Zapaženo je da omjer ci(Ca2+)/ ci(C2O42-), c(citrat) i način miješanja značajno utječu na morfologiju COD kristala

Abstract (english)

In this Thesis, systematic research of spontaneous precipitation of calcium oxalate at three different complexities (simple, NaCl and artificial urine system) and two initial pH (pHi = 5.0 and 9.0) was conducted. In all investigated systems, dominant precipitation of calcium oxalate monohydrate (COM) was observed, except in artificial urine system at pHi = 9.0, ci(C2O42-) ≥ 6.0 mmol dm-3 and ci(Ca2+) ≥ 7.5 mmol dm-3 where precipitation of a mixture of calcium oxalate monohydrate (COM) and calcium oxalate dihydrate (COD) occured. The influence of seven amino acids (nonpolar - Ala, Phe; polar - Gly, Ser, Cys and charged - His, Asp) on spontaneous pecipitation of calcium oxalate in hyperoxaluria conditions (ci(Ca2+) = 7.5 mmol dm-3 and ci(C2O42-) = 6.0 mmol dm-3) was studied in order to elucidate their role in precipitation of calcium oxalate hydrate. In all three investigated systems, significant influence of studied amino acids on morphology and crystal growth of COM was noted. The changes were more pronounced at pHi = 9.0. Only in the artificial urine system at pHi = 9.0, the change in composition was observed, polar aminoacids and nonpolar Phe inhibit precipitation of COM.
In this Thesis, COD was synthesized for the first time with ultrasonic mixing. Crystal seed of COD in a shape of proper tetragonal bipramids with prism was prepared in ultrasonically mixed system: ci(Ca2+) = 9.00 mmol dm-3, ci(C2O42-) = 2.33 mmol dm-3 and c(citrate) = 9.00 mmol dm-3 (ci(Ca2+)/ci(C2O42-) = 3.86). It was observed that the ci(Ca2+)/ ci(C2O42-) ratio, c(citrate) and the way of mixing singnificantlly influenced morphology of COD crystals.

• Keywords: hiperoksalurija; kalcijev oksalat monohidrat; kalcijev oksalat dihidrat; spontano taloženje; ultrazvučno miješanje; aminokiseline
• Keywords (english): hyperoxaluria; calcium oxalate monohydrate; calcium oxalate dihydrate; spontaneous precipitation; ultrasonic agitation; amino acids
• Language: croatian
• URN:NBN: urn:nbn:hr:217:491414
• Study programme: Title: Doctoral study in chemistry; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje kemija (doktor/doktorica znanosti, područje prirodnih znanosti, polje kemija)
• Type of resource: Text
• Extent: 126 str. ; 30 cm
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2018-09-25
• Created on: 2018-05-15 09:39:22