Karakterizacija ponavljajućih sljedova DNA u dalmatinskom buhaču (Tanacetum cinerariifolium (Trevir.) Sch. Bip.)

master's thesis

• Title (english): Characterization of repetitive DNA families in dalmatian pyrethrum (Tanacetum cinerariifolium (Trevir.) Sch. Bip.)
• Author: Ana Skuhala
• Mentor: Višnja Besendorfer (mentor)
• Committee member: Višnja Besendorfer (predsjednik povjerenstva)
• Committee member: Vesna Petrović Peroković (član povjerenstva)
• Committee member: Ines Radanović (član povjerenstva)
• Committee member: Draginja Mrvoš-Sermek (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2018-09-14-09-14, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Ponavljajuće DNA su brzo evoluirajuće te je njihova različitost vrlo važna u određivanju vrsta. Novija istraživanja pokazuju da su važne pri regulaciji diobe stanica te da utječu na veličinu i organizaciju genoma. Usprkos navedenom značaju, ponavljajuće DNA su još uvijek nedovoljno istražene. Dosadašnja istraživanja pokazala su da je rod Tanacetum zanimljiv evolucijski model obzirom na veliku varijabilnost u položaju ribosomskih gena među vrstama. Dalmatinski buhač je Hrvatski endem i ima veliku važnost u ekološkoj poljoprivredi. Bez obzira na vrlo važnu primjenu i relativno velike kromosome koji olakšavaju citogenetička istraživanja, dalmatinski buhač je citogenetički neistražena vrsta. U ovom istraživanju odredili smo položaj i organizaciju satelitne, ribosomske i telomerne DNA na kromosomima dalmatinskog buhača. U ovom radu istražen je položaj na kromosomima tri satelitne porodice TcSAT1, TcSAT2 i TcSAT3. Porodice TcSAT1 i TCSAT2 smještene su subtelomerno na kromosomima, dok je TcSAT3 smještena intersticijski na dva akrocentrična kromosmska para. Utvrđena su intersticijska telomerna ponavljanja (ITS) koja se smatraju evolucijski zastarjelim razmještajem na kromosomu. Metodom FISH dokazano je da genom buhača sadrži rijetku L-konfiguraciju 35S/5S rDNA. Provedeno istraživanje ukazuje na plastičnost genoma dalmatinskog buhača te da su subtelomerna područja najdinamičniji dio genoma dalmatinskog buhača.

Abstract (english)

Repetitive DNAs represent the most evolving DNA and their diversity is very important for determination of species. Resent research showed that they are involved in the regulation of cell division, determination of the genome size and organization. Despite its significance, repetitive DNA is poorly investigated. Genus Tanacetum is interesting evolutionary model because of great variability in localization of rRNA genes among species. Dalmatian pyrethrum is endemic to Croatia and has important role in ecological agriculture. Regardless of important role in agriculture and relatively large chromosomes that facilitate cytogenetic research, dalmatian pyrethrum is cytogenetically poorly investigated. Our study aims to identify organization of repetitive DNA in the T. cinereriifolium genome using FISH method. Three satellite DNA families were described in T. cinerariifolium TcSAT1, TcSAT2 I TcSAT3. TcSAT1 and TcSAT2 satellite DNA families were heterogeneously distributed among different chromosome ends, while TcSAT3 family was exclusively located intercalary on the longer arm of two acrocentric chromosome pairs. FISH analysis has identified interstitial telomeric repeats which are likely evolutionary relics of chromosomal rearrangements. T. cinerariifolium exhibited an unusual linked rDNA configuration (L-type). Altogether our data highlights the genome plasticity of T. cinerariifolium and confirms that subtelomeres represent one of the most dynamic regions in the genome.

• Keywords: dalmatinski buhač T. cinerariifolium; ponavljajuće DNA; FISH; L-konfiguracija 35S/5S rDNA
• Keywords (english): Dalmatian pyrethrum; T. cinerariifolium; L-type arrangement 35S/5S rDNA; repetitive DNA
• Language: croatian
• URN:NBN: urn:nbn:hr:217:741927
• Study programme: Title: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2020-09-14
• Created on: 2018-11-09 11:17:04