Određivanje arsena u biljnim ekstraktima metodama ETAAS i ICP-AES

master's thesis

• Title (english): Determination of arsenic in herbal extracts by ETAAS and ICP-AES methods
• Author: Sanja Alispahić
• Mentor: Sanda Rončević (mentor)
• Committee member: Sanda Rončević (predsjednik povjerenstva)
• Committee member: Biserka Prugovečki (član povjerenstva)
• Committee member: Morana Dulić (član povjerenstva)
• Committee member: Predrag Novak (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Chemistry); Zagreb
• Defense date and country: 2018-09-17, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry

Abstract

U ovom su se radu ispitivale mogućnosti određivanja ukupnih anorganskih specija arsena iz uzoraka
biljke Erodium cicutarium koja se koristi u tradicionalnom liječenju infekcija. Nakon primjene različitih
analitičkih postupaka obrade praškastog biljnog materijala, sadržaj arsena analizirao se metodom atomske
emisijske spektrometrije uz induktivno spregnutu plazmu (ICP-AES). Ispitane su i mogućnosti elektrotermičke
atomske apsorpcijske spektrometrije (ETAAS) u analizi pripravljenih otopina uzoraka uz postupke prethodne
redukcije i generiranja hidrida. Prethodna priprema uzorka temeljila se na razgradnji proteinske matrice u
koncentriranoj klorovodičnoj kiselini ili u koncentriranoj dušičnoj kiselini i vodikovom peroksidu pomoću
ultrazvučne kupelji ili sustava za mikrovalnu razgradnju. Kontrola točnosti analitičkih postupaka rađena je uz
certificirane referentne materijale biljaka s deklariranim ukupnim sadržajem arsena (NIST CRM 1573a. i NIST
CRM 1570a). Zadovoljavajuća točnost (<1.5%) postignuta je metodom ETAAS. Dobiveni podaci pokazali su
pouzdanost mjerenja metodom ETAAS, dok je analiza metodom ICP-AES pokazala veliki rasap mjerenih
vrijednosti. Raspon koncentracija arsena u uzorcima biljnog praha mjeren je u granicama 88-345 ng g-1.

Abstract (english)

The possibilities for determination of inorganic arsenic species from samples of a plant Erodium cicutarium,
which is used in traditional healing of infections, are described in this work. After the usage of different
analytical procedures for processing powdered plant material, content of arsenic was determined by inductively
coupled plasma atomic emission spectrometry (ICP-AES). The possibilites of electrothermal atomic absoption
spectrometry (ETAAS) for the analysis of sample solutions together with the prior procedures of reduction and
hydride generation, were also investigated. Sample preparation was performed by digestion of protein matrix in
concentrated hydrochloric or nitric acid together with ultrasound bath or microwave digestion system. Accuracy
of analytical procedures was controled by the standard reference materials of plants with declared total amount
of arsenic (NIST CRM 1573a. i NIST CRM 1570a). Acceptable accuracy (<1,5%) was accomplished by ETAAS
in contrast to ICP-AES which has shown a very wide range of measured values. Determinated concentrations of
arsenic in powdered plant materials were in the range of 88-345 ng g-1.

• Keywords: arsen; biljni ekstrakt; ETAAS; ICP-AES
• Keywords (english): arsenic; herbal extracts; ETAAS; ICP-AES
• Language: croatian
• URN:NBN: urn:nbn:hr:217:498255
• Study programme: Title: Graduate university studies in chemistry; specializations in: Research programme; Course: Research programme; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2020-09-17
• Created on: 2018-11-16 10:09:31