Učinak olova na fotosintetsku učinkovitost i pigmente u lišajeva Flavoparmelia caperata (L.) Hale i Evernia prunastri (L.) Ach

master's thesis

• Title (english): The effect of lead on photosynthetic efficiency and pigments in the lichens Flavoparmelia caperata (L.) Hale and Evernia prunastri (L.) Hale
• Author: Marija Čuček
• Mentor: Mirta Tkalec (mentor)
• Committee member: Mirta Tkalec (predsjednik povjerenstva)
• Committee member: Ana Galov (član povjerenstva)
• Committee member: Sandra Hudina (član povjerenstva)
• Committee member: Renata Šoštarić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2017-02-24, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Lišajevi su kozmopolitski organizmi i izvrsni bioindikatori zagađenja zraka. Sposobni su
bioakumulirati teške metale, a toksičnost metala ovisi o kemijskim i fizikalnim faktorima. Cilj
istraživanja je bio odrediti osjetljivost vrsta Evernia prunastri (L.) Ach i Flavoparmelia
caperata (L.) Hale na izlaganje olovu te utvrditi kako pH vrijednost otopine (4 i 6) i vrsta
otopine (destilirana voda ili Hoaglandova otopina), modificiraju učinak olova nakon 1., 7. i
14. dana. U obje vrste tretman olovom je snizio QYmax (maksimalni kvantni prinos
fotosustava II) nakon 1., 7. i 14. dana, što ukazuje na smanjenu učinkovitost fotosinteze. U
vrste E. prunastri Hoaglandova otopina smanjila je negativan učinak olova osobito pri pH
vrijednosti 6, dok je u vrste F. caperata Hoaglandova otopina smanjila negativan učinak
olova tek nakon 14. dana. U obje vrste lišajeva tretman olovom je uglavnom snizio
koncentraciju klorofila a i klorofila b te karotenoida tek nakon 14 dana tretmana. Niži
koeficijent feofitinizacije ukazuje na degradaciju klorofila a uslijed tretmana olovom.
Hoglandova otopina smanjila je negativan učinak olova na pigmente ali u vrste E. prunastri to
se očitovalo kod pH vrijednosti 6, a u vrste F. caperata pri pH vrijednosti 4. U obje vrste lišaja
tretman olovom je povisio razinu lipidnih peroksida osobito pri pH vrijednosti 6.
Hoaglandova otopina pH vrijednosti 6 smanjila je negativan učinak olova na oštećenje
membrana. Na temelju rezultata može se zaključiti da su obje vrste lišaja osjetljive na
tretman olovom osobito pri pH vrijednosti 6 što je posljedica bolje dostupnosti olova pri toj
pH vrijednosti. U obje vrste prisutnost iona iz Hoaglandove otopine smanjila je negativan
učinak olova ali je efikasnost ovisila o pH vrijednosti otopine koja je bila različita za svaku
vrstu.

Abstract (english)

Lichens are cosmopolitan organisms and great bioindicators of air pollution. They
bioaccumulate heavy metals whose toxicity depends on chemical and physical factors. Aim
of this research was to determine sensitivity of Evernia prunastri (L) Ach and Flavoparmelia
caperata (L) Hale to lead exposure and to determine if changes in pH value (4 or 6) and
solution composition (Hoaglnd solution or destilled water) modulate lead toxicity after 1., 7.
and 14. days of exposure. Both species showed lower QYmax (the largest light quantum
efficiency of photosystem II) when tretated with lead after 1., 7. and 14. days of exposure
which indicates decrease in photosynthetic efficiency. Hoagland solution reduced negative
effect of lead especially at pH value 6 in E. prunastri, while in F. caperata it reduced negative
effect of lead only after 14. days of exposure. In both species lead treatment mainly
decreased chlorophyll a, chlorophyll b and carotenoid concentrations only after 14. days of
treatment. Decreased phaeophytinization coefficient indicates chlorophyll a degradation
due to lead treatment. Hoagland solution decreased negative effect of lead on pigments, in
E. prunastri at pH value 6 and in F. caperata at pH value 4. In both species lead tretment
increased concentration of lipid peroxides especially at pH value 6. Hoagland solution at pH
value 6 decreased negative effect of lead on membrane damage. These results confirmed
sensitivity of both lichen species to lead treatment, most notably at pH value 6 when lead
availability is the highest. In both species availability od ions from Hoagland solution
decreased negative effect od lead, but it depended on the pH value of solution and was
different for each lichen species.

• Keywords: Flavoparmelia caperata; Evernia prunastri; olovo; teški metali; fotosinteza; pigmenti; lipidna peroksidacija; lišajevi
• Keywords (english): Flavoparmelia caperata; Evernia prunastri; lead; heavy metals; photosyntesis; pigments; lipid peroxidation; lichens
• Language: croatian
• URN:NBN: urn:nbn:hr:217:007852
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-04-20 10:01:58