Utjecaj ekstrakata biljaka Moltkia petraea i Micromeria croatica na zaštitu telomera, stanično starenje i proliferaciju stanica MJ90

master's thesis

• Title (english): Influence of Moltkia petraea i Micromeria croatica plant extracts on telomere, cellullar aging and proliferation of MJ90 cells
• Author: Matea Cedilak
• Mentor: Ivica Rubelj (mentor)
• Mentor: Maja Matulić (mentor)
• Committee member: Maja Matulić (predsjednik povjerenstva)
• Committee member: Renata Šoštarić (član povjerenstva)
• Committee member: Ivančica Ternjej (član povjerenstva)
• Committee member: Inga Marijanović (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2015-02-16, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Telomere imaju glavnu ulogu u zaštiti strukture kromosoma. Također štite krajeve kromosoma od međusobnih fuzija te imaju ulogu „brojača dioba“ jer se sa svakom diobom stanice one skraduju. Kada telomere dostignu kritičnu duljinu, stanica ulazi u permanentni zastoj staničnog ciklusa (senescencija), stoga su telomere najbitniji regulator staničnog starenja. Zaštitom telomera od oštedenja može se spriječiti naglo skradivanje telomera, kao i odgoditi ulazak stanice u senescenciju. U ovom sam radu istražila utjecaj ekstrakata biljaka Micromeria croatica i Moltkia petraea na zaštitu telomera te time i na stanični rast i starenje. Stanice su tretirane ekstraktima kroz dulji period rasta u staničnoj kulturi te je za određivanje udjela starih stanica korištena metoda bojanja SA-β-gal, a za određivanje udjela stanica u proliferaciji obilježavanje DNA s BrdU. Stanicama je također izmjerena duljina telomera metodom hibridizacije po Southernu. Stanice tretirane ekstraktima pokazale su manji udio starih stanica kao i dulje telomere u odnosu na kontrolne stanice te sam zaključila da ekstrakti ovih biljaka usporavaju stanično starenje, vjerojatno putem zaštite proteina ili DNA stanice.

Abstract (english)

The main role of telomeres is the protection of the chromosome structure. They also protect the chromosome ends from fusions, and since they shorten with every cell division they serve as counters of population doublings. Once the telomeres reach their critical length, the cell enters permanent and irreversible cell cycle arrest called senescence which makes them the most important regulators of the cellular ageing. By protecting the telomeres we could prevent their abrrupt shortening and also postpone the cell senescence. Effect of the plant extracts Micromeria croatica and Moltkia petraea on the protection of telomeres and therefore on the cell growth and ageing, was the main goal of this research. Cells were treated with the extracts for a longer period of time in the cell culture. SA-β-gal staining was used to evaluate the cell senescence, and BrdU labelling to examine cell proliferation. The telomere length was measured using Southern blot analysis. Cells treated with plant extracts exibited lower number of senesced cells and slightly longer telomeres compared to the non-treated control cells. This supports the conclusion that the extracts inhibit cellular aging, probably through the protection of the cell DNA and protein structures.

• Keywords: skradenje telomera; senescencija; ekstrakti M. croatica i M. petraea
• Keywords (english): telomere shortening; senescence; M. croatica and M. petraea extracts
• Language: croatian
• URN:NBN: urn:nbn:hr:217:232942
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-04-21 09:20:19