Morfološka i genetička raznolikost populacija invazivnog raka Pacifastacus leniusculus (Dana, 1852) u Hrvatskoj

master's thesis

• Title (english): Morphological and genetic diversity of populations of the invasive crayfish Pacifastacus leniusculus (Dana, 1852) in Croatia
• Author: Tatjana Mijošek
• Mentor: Ivana Maguire (mentor)
• Committee member: Ivana Maguire (predsjednik povjerenstva)
• Committee member: Ana Galov (član povjerenstva)
• Committee member: Božena Mitić (član povjerenstva)
• Committee member: Jasna Lajtner (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2015-11-19, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Signalni rak (Pacifastacus leniusculus) je autohton za područje Sjeverne Amerike. Introduciran je na područje Europe i Japana, ali se ubrzo nakon unošenja proširio kao invazivna vrsta. U Hrvatskoj je rasprostranjen u rijekama Muri i Korani gdje je u kompeticiji s autohtonim vrstama te je prijenosnik patogena (Aphanomyces astaci); uzročnika bolesti koja je smrtonosna za autohtone vrste rakova. Budući da se malo zna o genetičkoj raznolikosti vrste, u ovom istraživanju su korišteni mikrosatelitni lokusi i mitohondrijski geni za 16S rRNA s ciljem utvrđivanja međusobnih filogenetskih odnosa proučavanih populacija kao i njihova odnosa prema već istraženim populacijama ove vrste u svijetu. Analizirane su i morfometrijske značajke kako bi se utvrdilo postoje li značajne razlike između populacija i podudaraju li se rezultati morfometrijskh analiza s rezultatima genetskih analiza. Rezultati pokazuju da je raznolikost signalnog raka nešto veća u rijeci Muri, nego u Korani, ali niti jedna metoda jasno ne odvaja dvije populacije. Raznolikost na razini Hrvatske nešto je manja u odnosu na već istražene skandinavske i zapadnoeuropske zemlje. Rezultati ovog rada također pridonose spoznajama o ovoj vrsti kao i procjeni njezinog invazivnog uspjeha.

Abstract (english)

Signal crayfish (Pacifastacus leniusculus) is a native to North America. It was introduced to Europe and Japan but it soon spread as an invasive species. In Croatia, it is widespread in the rivers Mura and Korana where it competes with native spieces. Moreover, it may be an Aphanomyces astaci carrier, a pathogen causing a disease lethal to native crayfish spieces. Since the knowledge on the genetic diversity of this species is limited, microsatellite markers and mitochondrial gene 16S rRNA analyses were used to explore the phylogenetic relations among Croatian populations as well as their relation to other already studied populations. Morphometric characteristics were studied to determine if there are significant differences among populations and if these are concordant with the genetic analyses results. Results showed that the genetic diversity of Pacifastacus leniusculus is slightly higher within the Mura population compared to the Korana population, but no clearly seperation of these two populations wer obtained. Genetic diversity of this species within Croatia is slightly lower in comparison to already investigated Scandinavian and West European countries. This study contributes to the knowledge on signal crayfish, its characteristics, genetic diversity as well as the possible estimation of its invasive success.

• Keywords: invazivna vrsta; mikrosatelitni lokusi; 16S rRNA; morfometrijske značajke; filogenetski odnosi; invazivni uspjeh
• Keywords (english): invasive species; microsatellite markers; 16S rRNA; morphometric characteristics; phylogenetic relations; invasive success
• Language: croatian
• URN:NBN: urn:nbn:hr:217:404576
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-04-24 07:49:27