Zajednički učinak okratoksina A i citrinina na oksidacijski stres u štakora

master's thesis

• Title (english): The effect of simultaneous treatment with ochratoxin A and citrinin on oxidative stress in rat
• Author: Lidija Domitrović
• Mentor: Maja Peraica (mentor)
• Mentor: Nada Oršolić (mentor)
• Committee member: Nada Oršolić (predsjednik povjerenstva)
• Committee member: Maja Peraica (član povjerenstva)
• Committee member: Mirta Tkalec (član povjerenstva)
• Committee member: Vesna Benković (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2013-02-06, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Mikotoksini okratoksin A (OTA) i citrinin (CIT) često su istovremeno prisutni u uzorcima hrane. Uz dokazano nefrotoksično, hepatotoksično i karcinogeno djelovanje, povezuju se s bolešću balkanska endemska nefropatija (BEN). Mehanizam njihovog staničnog djelovanja neki autori povezuju sa stvaranjem oksidacijskog stresa. Cilj ovog istraživanja bio je utvrditi je li oksidacijski stres mehanizam toksičnog učinka OTA i CIT na pokusnim životinjama, te mijenja li se taj učinak ako su primijenjeni istovremeno. Istraživanje je provedeno na odraslim mužjacima štakora soja Wistar. U skupini izlaganoj OTA (250 μg/kg t.m. dnevno, kroz tri tjedna) izmjereno je značajno smanjenje koncentracije reduciranog glutationa (GSH) u bubrezima te povećanje koncentracije malondialdehida (MDA) u jetri. U skupini izlaganoj CIT (20 mg/kg t.m., dva dana) nije bilo značajnih promjena. U skupini izlaganoj OTA i CIT (250 μg/kg t.m. OTA dnevno, kroz tri tjedna + 20 mg/kg t.m. CIT zadnja dva dana) izmjereno je značajno smanjenje koncentracije GSH u mozgu te povećanje koncentracije MDA u jetri i bubrezima. Primijećen je aditivan učinak OTA i CIT na povećanje koncentracije MDA u jetri. Takvi rezultati ukazuju na moguću zajedničku ulogu OTA i CIT prilikom izazivanja BEN.

Abstract (english)

Mycotoxins ochratoxin A (OTA) and citrinin (CIT) are often simultaneously present in food samples. Due to their nephrotoxic, hepatotoxic and carcinogenic effects, they are brought in connection with a disease called Balkan endemic nephropathy (BEN). Mechanism of their cellular activity is related with formation of oxidative stress by some authors. The objective of this research was to determine if oxidative stress is a mechanism of toxic effect of OTA and CIT on experimental animals, and whether that effect changes if they are applied simultaneously. The research was conducted on male Wistar rats. In the group treated with OTA (250 µg/kg b.m. per day, for three weeks), a significant decrease in glutathione (GSH) concentration in kidneys and increase of malondialdehyde (MDA) concentration in liver were measured. In the group treated with CIT (20 mg/kg b.m., two days) there were no significant changes. In the group treated with OTA and CIT (250 µg/kg b.m. OTA per day, for three weeks + 20 mg/kg b.m. CIT last two days) a significant decrease in GSH concentration in brain and increase of MDA concentration in liver and kidneys were found. Additive effect of OTA and CIT on increase of MDA concentration in liver was noticed. Such results indicate to the possible mutual role of OTA and CIT in causing BEN.

• Keywords: glutation (GSH); malondialdehid (MDA); štakori; bubreg; jetra; mozak
• Keywords (english): glutathione (GSH); malondialdehyde (MDA); rats; kidney; liver; brain
• Language: croatian
• URN:NBN: urn:nbn:hr:217:521309
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-04-27 07:38:06