Karakterizacija potencijalnih markera neuroloških poremećaja spektrometrijom masa

master's thesis

• Title (english): Characterization of potential markers for neurological disorders by mass spectrometry
• Author: Ružica Šoić
• Mentor: Renata Kobetić (mentor)
• Committee member: Sanda Rončević (predsjednik povjerenstva)
• Committee member: Vesna Petrović Peroković (član povjerenstva)
• Committee member: Ivica Đilović (član povjerenstva)
• Committee member: Iva Juranović Cindrić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Chemistry); Zagreb
• Defense date and country: 2020-11-27, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry

Abstract

svrhu pronalaska potencijalnih markera za rano otkrivanje neuroloških poremećaja provedena je karakterizacija cijaninskih boja, kao i analiza njihovog vezanja s proteinom IbpA te nisu pronađeni uvjeti i radni parametri pogodni za vezanje. Okarakteriziran je i tripeptid fenilalanil-fenilalanil-alanin (FFA) te je ispitivan utjecaj biološke matrice odnosno, pokazano je da se FFA s Cu2+ veže u molarnim omjerima 1:1 i 2:1 dok su za kompleks FFA-Zn2+ ti omjeri: 1:1, 2:1, 3:1, 4:1. Analiza sastava metala cerebrospinalnog likvora ukazuje na to da su smanjene vrijednosti ukupnog željeza potencijalni indikator demijelinizirajućih bolesti.
Spektrometrija masa (MS) odabrana je kao metoda za karakterizaciju potencijalnih markera neuroloških poremećaja i analizu sastava metala cerebrospinalnog likvora s obzirom da se primjenom raznih MS tehnika može brzo i efikasno okarakterizirati veliki broj različitih analita. Spektrometrija masa uz ionizaciju elektroraspršenjem (ESI-MS) odabrana je za karakterizaciju cijaninskih boja i tripeptida FFA, dok je analiza udjela metala u cerebrospinalnom likvoru provedena spektrometrijom masa uz induktivno spregnutu plazmu (ICP-MS).

Abstract (english)

In order to find potential markers for early detection of neurological disorders, cyanine dyes were characterized. Suitable conditions and operating parameters for analysis of cyanine dye-IbpA protein interactions were not found. Also, tripeptide phenylalanine-phenylalanine-alanine (FFA) was characterized and analysis of the influence of biological matrix shows that FFA binds to Cu2+ in molar ratios: 1:1 and 2:1, while these ratios for complex FFA-Zn2+ are: 1:1, 2:1, 3:1 and 4:1. Furthermore, analysis of total metal concentrations in cerebrospinal fluid (CSF) shows that lower concentrations of iron can be an indicator for demyelinating diseases.
Mass spectrometry (MS) is selected as a method for the characterization of potential markers for neurological disorders and analysis of selected metals in CSF, because different MS techniques are suitable for the fast and efficient characterization of a large number of analytes. Cyanine dyes and tripeptide FFA were characterized by electrospray ionization mass spectrometry (ESI-MS). The content of metals in CSF was analysed by inductively coupled plasma mass spectrometry (ICP-MS).

• Keywords: cerebrospinalni likvor; cijaninske boje; fenilalanil-fenilalalnil-alanin; metali; spektrometrija masa; neurološki poremećaji
• Keywords (english): cerebrospinal fluid; cyanine dyes; phenylalanine-phenylalanine-alanine; metals; mass spectrometry; neurological disorders.
• Language: croatian
• URN:NBN: urn:nbn:hr:217:721505
• Study programme: Title: Graduate university studies in chemistry; specializations in: Research programme; Course: Research programme; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-12-11 10:59:44