Genotipizacija rotavirusa A iz divljih životinja s područja Republike Hrvatske

master's thesis

• Title (english): Genotyping of Rotavirus A from wild animals in Croatia
• Author: Daniel Čolić
• Mentor: Dragan Brnić (mentor)
• Mentor: Silvija Černi (sumentor)
• Committee member: Silvija Černi (predsjednik povjerenstva)
• Committee member: Sofia Ana Blažević (član povjerenstva)
• Committee member: Rosa Karlić (član povjerenstva)
• Committee member: Marin Ježić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2021-02-19, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Rotavirus A (RVA) je dvolančani RNA virus iz roda Rotavirus. Uzročnik je akutnog
gastroenteritisa kod sisavaca i ptica i karakterizira ga visoka prevalencija, patogenost i genska
raznolikost. Genom RVA sastoji se od 11 dvolančanih RNA segmenata. Sustav klasifikacije
RVA temelji se na genotipizaciji segmenata VP7 (genotip G) i VP4 (genotip P). Važno
svojstvo RVA je potencijal međuvrsnog prijenosa i zoonotski potencijal, koji je najčešće
vezan za pojavu preslagivanja segmenata genoma. Cilj ovog istraživanja bio je odrediti
genotipove RVA koji cirkuliraju u populacijama crvenih lisica (Vulpes vulpes), europskih
čagljeva (Canis aureus moreoticus), divljih svinja (Sus scrofa), galebova klaukavaca (Larus
michahellis) i riječnih galebova (Larus ridibundus) s područja Republike Hrvatske. Metodom
RT-PCR u stvarnom vremenu dokazana je prisutnost genoma RVA u 11% (58 od 523)
obrađenih uzoraka. Sekvencije su utvrđene sekvenciranjem metodom po Sangeru, a
genotipizacijom koja je uslijedila utvrđeno je ukupno sedam različitih genotipova G, pet
genotipova P, tri genotipa E i jedan genotip I. Rezultati genotipizacije ukazuju na iznimnu
raznolikost i heterogenost RVA kod divljih životinja s područja RH, a filogenetska analiza
pokazuje potencijal međuvrsnog prijenosa i potkrepljuje važnost daljnjih istraživanja RVA
kod divljih životinja.

Abstract (english)

Rotavirus A is a double-stranded RNA virus from the genus Rotavirus. It causes acute
gastroenteritis in mammals and birds, and is characterized by high prevalence, pathogenicity
and genetic diversity. The genome of RVA consists of 11 double-stranded RNA segments.
The RVA classification system is based on the genotyping of segments VP7 (genotype G) and
VP4 (genotype P). An important feature of RVA is the potential of interspecies transmission
and zoonotic potential, which is most often associated with the occurrence of genetic
reassortment. This study aimed to determine genotypes of RVAs circulating in populations of
red foxes (Vulpes vulpes), European jackals (Canis aureus moreoticus), wild boars (Sus
scrofa), yellow-legged gulls (Larus michahellis) and black-headed gulls (Larus ridibundus) in
Croatia. The presence of RVA genome in 11% (58/523) of analyzed samples was proved by
real-time RT-PCR. Sequences were obtained by Sanger sequencing, and subsequent
genotyping detected seven different genotypes G, five genotypes P, three genotypes E and one
genotype I. The results indicate exceptional diversity and heterogeneity of RVA from wild
animals in Croatia, and phylogenetic analysis demonstrates the potential for interspecies
transmission and supports the importance of further research on RVA in wildlife.

• Keywords: rotavirus A; genotipovi; molekularna filogenija; međuvrsni prijenos
• Keywords (english): Rotavirus A; genotype; molecular phylogenetics; interspecies transmission
• Language: croatian
• URN:NBN: urn:nbn:hr:217:900539
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2021-09-19
• Created on: 2021-03-02 11:40:13