Funkcionalni i molekularni učinci prehrane bogate mastima u štakora s promijenjenom homeostazom serotonina

doctoral thesis

• Title (english): Functional and molecular effects of high-fat diet: studies in sublines of rats with constitutionally altered serotonin homeostasis
• Author: Petra Baković
• Mentor: Jasminka Štefulj (mentor)
• Committee member: Sofia Ana Blažević (predsjednik povjerenstva)
• Committee member: Lipa Čičin-Šain (član povjerenstva)
• Committee member: Maja Jazvinšćak Jembrek (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2021, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology
• Universal decimal classification (UDC): 577 - Biochemistry. Molecular biology. Biophysics

Abstract

Održavanje energetske ravnoteže je složena fiziološka funkcija čija disregulacija može rezultirati pretilošću i metaboličkim poremećajima poput dijabetesa. Ovim radom željelo se doprinijeti boljem razumijevanju uloge serotonina (5HT) u mehanizmima regulacije energetske homeostaze, korištenjem sublinija štakora s konstitucijski povišenom (5HT-visoka) ili sniženom (5HT-niska) aktivnošću serotonina. Životinje iz obiju sublinija podvrgnute su visokokaloričnoj prehrani s visokim udjelom masti te je uspoređen njihov funkcionalni i molekularni odgovor na takav metabolički izazov. U skladu s prethodnim nalazima, životinje iz 5HT-visoke sublinije su u uvjetima standardne prehrane imale lošije metaboličko zdravlje od 5HT-niskih životinja. Međutim, 5HT-niske životinje su u odgovoru na visokokaloričnu prehranu pokazale veću sklonost debljanju, slabiju regulaciju glikemije, jači razvoj rezistencije na inzulin te veće promjene u ekspresiji različitih gena u hipotalamusu i bijelom masnom tkivu. Dobiveni rezultati pokazuju da je konstitucijski snižena aktivnost serotonina povezana s većom osjetljivošću na štetne učinke visokokalorične prehrane.

Abstract (english)

Maintenance of energy balance is a complex physiological function, dysregulation of which can lead to obesity and metabolic disorders such as diabetes. Here, we aimed to contribute to a better understanding of the role of serotonin (5HT) in the mechanisms regulating energy homeostasis, by using sublines of rats with constitutionally increased (5HT-high) or decreased (5HT-low) activity of 5HT. Animals from both sublines were subjected to a high-calorie, high-fat diet and compared for their functional and molecular response to this metabolic challenge. Consistent with previous findings, when administered conventional diet, animals from the 5HT-high subline had poorer metabolic health than animals from 5HT-low subline. However, in response to a high-fat diet, 5HT-low animals showed a greater tendency to gain weight, poorer glycemic control, higher development of insulin resistance, and more pronounced changes in the expression of various metabolic genes in the hypothalamus and adipose tissue. The obtained results suggest that constitutionally decreased activity of 5HT is associated with a higher susceptibility to adverse effects of a high-calorie diet.

• Keywords: pretilost; serotonin; visokokalorična prehrana; animalni model; Wistar-Zagreb 5HT štakor
• Keywords (english): obesity; serotonin; high-fat diet; animal model; Wistar-Zagreb 5HT rat
• Language: croatian
• URN:NBN: urn:nbn:hr:217:800927
• Promotion: 2021
• Study programme: Title: Biology; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2023-07-23
• Created on: 2021-09-16 11:17:31