Perlit kao nosač bakterija u reaktorima za proizvodnju bioplina

master's thesis

• Title (english): Perlite as a bacterial carrier in biogas producing reactors
• Author: Ivana Paladin
• Mentor: Tomislav Ivanković (mentor)
• Committee member: Tomislav Ivanković (predsjednik povjerenstva)
• Committee member: Nenad Judaš (član povjerenstva)
• Committee member: Mirela Sertić Perić (član povjerenstva)
• Committee member: Draginja Mrvoš-Sermek (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2021-09-28, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Bioplin nastaje procesom anaerobne pretvorbe organskih materijala uz prisustvo anaerobnih organizama. Jedno od biotehnoloških rješenja za proizvodnju bioplina su reaktori s aktivnim muljem koji koriste poljoprivrednu energetsku kulturu Miscanthus x giganteus kao alternativnu sirovinu kukuruznoj silaži. Cilj rada je bioaugmentirati reaktore za proizvodnju bioplina s ciljem povećanja njihove učinkovitosti. Bakterije koje smo uspjeli izolirati iz aktivnog mulja koristile su M. giganteus kao jedini izvor ugljika. Nakon izolacije bakterije uspješno su se imobilizirale na perlit biofilmom te tako su tvorile biočestice. Postavljanjem 9 različitih bioreaktora i njihovim praćenjem proizvodnje bioplina ukazalo se da nema značajnih razlika u proizvodnji bioplina između reaktora s biočesticama, bakterijskom suspenzijom i aktivnog mulja s dodatkom
M. giganteus. Predlaže se daljnje i opširnije istraživanje s više replika te s različitim koncentracijama biočestica, da bi se vidjelo može li se povećati prinos bioplina u nekoj drugoj konfiguraciji, ili možda da se poveća stabilnost različitih bioreaktora.

Abstract (english)

Biogas is produced during the process of anaerobic conversion of organic materials in the presence of anaerobic organisms. A biotechnological solution for biogas production is active sludge reactor using the agricultural energy crop Miscanthus x giganteus as an alternative raw material to corn silage. The aim of this paper is to bioaugment production reactors biogas in order to increase their efficiency. Successfully isolating the bacteria from activated sludge required using M. giganteus as the only carbon source. After isolating the bacteria, they were successfully immobilized on perlite using biofilm and subsequently formed bioparticles. Setting 9 different bioreactors and monitoring biogas production indicated that there are no significant differences in biogas production between bioparticle reactors, bacterial suspension and activated sludge with added M. giganteus. The recommendation is to undertake further and more extensive research with more replications and different concentrations of bioparticles, in order to ascertain whether biogas can be increased in another configuration, or perhaps increase the stability of different types of bioreactors.

• Keywords: bioaugmentacija; Miscanthus x giganteus; imobilizacija; biočestice; aktivni mulj; biofilm
• Keywords (english): bioaugmentation; Miscanthus x giganteus; active sludge; immobilization; bioparticles; biofilm
• Language: croatian
• URN:NBN: urn:nbn:hr:217:732002
• Study programme: Title: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-10-05 12:52:10