Polielektrolitni višesloj kitozan/poli(akrilna kiselina): priprava, karakterizacija i antibakterijska svojstva

master's thesis

• Title (english): Polyelectrolyte multilayer chitosan/poly(acrylic acid): preparation, characterization and antibacterial properties
• Author: Luka Babić
• Mentor: Davor Kovačević (mentor)
• Committee member: Davor Kovačević (predsjednik povjerenstva)
• Committee member: Željka Soldin (član povjerenstva)
• Committee member: Marko Močibob (član povjerenstva)
• Committee member: Tajana Begović (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Chemistry); Zagreb
• Defense date and country: 2023-02-23, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry

Abstract

Polielektroliti su makromolekule koje se sastoje od ponavljajućih podjedinica koje se mogu
ionizirati u vodenoj otopini. Uzastopnom naizmjeničnom adsorpcijom polielektrolita različitih
naboja na supstrat, pomoću „sloj po sloj“ metode, nastaje polielektrolitni višesloj.
Polielektrolitni višeslojevi se mogu koristiti kao antibakterijski filmovi. Na adheziju bakterija
utječu hrapavost, naboj i hidrofilnost površine. U ovom radu istražena su svojstva
polielektrolitnog višesloja koji se sastoji od kitozana i poli(akrilne kiseline), te višeslojeva na
koje je adsorbiran lizozim. Rezultati mjerenja zeta potencijala i potencijala strujanja pokazali
su da je površina istraženih filmova negativno nabijena pri fiziološkom pH. Utvrđeno je da je
površina pripravljenih višeslojeva hidrofilna i približno jednake hrapavosti koja odgovara
glatkim površinama. Adhezija bakterija Pseudomonas aeruginosa i Staphylococcus aureus
istražena je pomoću pretražnog elektronskog mikroskopa te je ustanovljeno da su
antibakterijska svojstva filmova izraženija kada je lizozim zadnji sloj.

Abstract (english)

Polyelectrolytes are macromolecules comprised of repeating units that could be ionized in an
aqueous solution. Polyelectrolyte multilayers are prepared by alternating adsorption on a
substrate using „layer by layer“ method. Polyelectrolyte multilayers can be used as antibacterial
films. Bacterial adhesion depends on properties such as roughness, charge and hydrophilicity.
In this thesis, these properties of a polyelectrolyte multilayer consisting of chitosan and
poly(acrylic acid), as well as those with an added layer of lysozyme were studied. Zeta and
streaming potential results showed that the examined surfaces were negatively charged at
physiological pH. The surfaces of all prepared multilayers were hydrophylic and had similar
roughness which corresponds to smooth surfaces. Adhesion of bacteria Pseudomonas
aeruginosa and Staphylococcus aureus was studied using scanning electron microscopy and it
was shown that antibacterial properties were more pronounced in the case with lysozyme as the
terminating layer.

• Keywords: antiadheziv; baktericid; lizozim; polielektroliti; polielektrolitni višesloj
• Keywords (english): antiadhesive properties; bactericide; lysozyme; polyelectrolyte; polyelectrolyte multilayer
• Language: croatian
• URN:NBN: urn:nbn:hr:217:786751
• Project: Number: IPS-2020-01-6126; Title: Modulacija polifenolnog profila voća uslijed fizikalnih tretmana nakon branja; Title: Modulation of fruit polyphenolic profile by sustainable postharvest physical treatments; Leader: Davor Kovačević; Jurisdiction: Croatia; Funder: HRZZ; Funding stream: IPS
• Study programme: Title: Graduate university studies in chemistry; specializations in: Research programme; Course: Research programme; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2025-02-23
• Created on: 2023-03-31 08:27:58