Utjecaj položaja His6 privjeska na svojstva proteina, primjer adenilosukcinat-sintetaze iz bakterije Helicobacter pylori ; O lijekovima kroz predtercijarnu nastavu kemije

master's thesis

• Title (english): Effect of His6 tag position on protein properties, the example of adenylosuccinate synthease from Heliobacter pylori ; About drugs through pre-tertiary chemistry teaching
• Author: Marija Zora Mišković
• Mentor: Ivana Leščić Ašler (mentor)
• Mentor: Draginja Mrvoš-Sermek (mentor)
• Committee member: Draginja Mrvoš-Sermek (predsjednik povjerenstva)
• Committee member: Jasmina Rokov Plavec (član povjerenstva)
• Committee member: Mirela Sertić Perić (član povjerenstva)
• Committee member: Renata Matoničkin Kepčija (član povjerenstva)
• Committee member: Željka Soldin (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Granter: University of Zagreb; Faculty of Science; (Department of Chemistry); Zagreb
• Defense date and country: 2025-02-21, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry

Abstract

Za znanstvena istraživanja nužno je osigurati pročišćene proteine, što se često postiže dodavanjem specifičnih privjesaka poput histidinskog niza na proteinsku sekvencu. Enzim adenilosukcinat-sintetaza iz bakterije Helicobacter pylori, ključan u biosintezi purina, obilježen histidinskim privjeskom na svom N-kraju, pročišćen je afinitetnom kromatografijom i gel- filtracijom. Biokemijska karakterizacija obuhvatila je određivanje molekulske mase, izoelektrične točke, kinetičkih konstanti i... More stabilnosti enzima pri različitim uvjetima. Istraživanje je pokazalo da histidinski privjesak utječe na stabilnost i aktivnost enzima. Metodički dio rada predlaže nastavne aktivnosti za izbornu temu Znanost o materijalima u 4. razredu gimnazije, s naglaskom na integraciju kemijskih koncepata i kritičko promišljanje o kurikulumu. Inovativne metode poput interaktivnih lekcija i simulacija otkrivanja lijeka razmatrane su kako bi se unaprijedilo poučavanje i potaknulo kritičko promišljanje o uporabi lijekova i zdravstvenim rizicima. Less

Abstract (english)

For scientific research, it is necessary to provide purified proteins, which is often achieved by adding specific appendages such as a histidine tag to the protein sequence. The enzyme adenylosuccinate synthetase from the bacterium Helicobacter pylori, key enzyme in purine biosynthesis, having a His-tag on its N-terminus, was purified by affinity chromatography and gel filtration. Biochemical characterization included determination of molecular weight, isoelectric point, kinetic... More constants and enzyme stability under different conditions. Research has shown that the histidine tag affects the stability and activity of the enzyme. The methodological part of the work foresees teaching activities for the optional topic Materials Science in the 4th grade of high school, with an emphasis on the integration of chemical concepts and critical thinking about the curriculum. Innovative methods such as interactive lessons and drug discovery simulations were considered to enhance teaching and encourage critical thinking about drug use and health risks. Less

• Keywords: aktivnost enzima; enzimi; lijekovi; nastava kemije; stabilnost enzima
• Keywords (english): enzymes; enzyme activity; enzyme stability; medicines; teaching chemistry
• Language: croatian
• URN:NBN: urn:nbn:hr:217:158502
• Project: Number: IP-2019-04-6764; Title: Alosterički komunikacijski putevi u oligomernim enzimima; Title: Allosteric communication pathways in oligomeric enzymes; Acronym: ALOKOMP/ALOCOMP; Leader: Zoran Štefanić; Jurisdiction: Croatia; Funder: Hrvatska zaklada za znanost; Funding stream: Research Projects
• Study programme: Title: Biology and Chemistry Education-University integrated undergraduate and graduate programme; specializations in: Biology; Course: Biology; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: sveučilišni/a magistar/magistra edukacije biologije i kemije (sveučilišni/a magistar/magistra edukacije biologije i kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2025-03-21 11:46:37