Procjena toksičnosti vode rijeke Save korištenjem rakušca Gammarus fossarum i rašljoticalca Daphnia magna te kemijskom karakterizacijom vode

master's thesis

• Title (english): Toxicity assessment of the water from Sava River using amphipods Gammarus fossarum and cladocerans Daphnia magna and chemical characterization of water
• Author: Martina Drnić
• Mentor: Goran Klobučar (mentor)
• Committee member: Goran Klobučar (predsjednik povjerenstva)
• Committee member: Sandra Radić Brkanac (član povjerenstva)
• Committee member: Alan Moro (član povjerenstva)
• Committee member: Ivan Čanjevac (član povjerenstva)
• Committee member: Danijel Orešić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2019-06-17, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Kontinuirani industrijski i tehnološki napredak čovječanstva rezultira sve većim
brojem različitih onečišćivala prisutnih u okolišu zbog čega raste potreba za monitoringom
onečišćenja i utvrđivanjem eventualnih negativnih posljedica koje ono može prouzročiti. U
ovom su radu korištena dva standardizirana testa u svrhu procjene kvalitete vode rijeke Save.
Uzevši u obzir bitne uloge beskralježnjaka u slatkovodnom okolišu, slatkovodni rakovi
Gammarus fossarum (Koch, 1836) i Daphnia magna (Straus, 1820) korišteni su u ovom
istraživanju kao modelni organizmi zbog visoke osjetljivosti na onečišćenje vode, lakog
rukovanja i mogućnosti praćenja promjena ponašanja. Također, biokemijski parametri
(detoksikacijska aktivnost pomoću enzima glutation-S-transferaze (GST), oksidacijska
oštećenja uz mjerenje sadržaja malondialdehida (MDA)) koji se na njima mogu analizirati
dodatan su razlog izbora ovih modelnih organizama. Modelni su organizmi bili izloženi
filtriranim uzorcima vode s pet lokacija za koje su utvrđeni različiti stupnjevi onečišćenja
vode (Hrušćica > Rugvica > Jesenice > Lukavec > Jankomir). Prisutnost onečišćivala na ovim
je postajama utvrđena visokoprotočnom kemijskom analizom vode. Nakon izlaganja, testom
učinkovitosti hranjenja utvrđena je smanjena stopa hranjenja, aktivnost GST bila je povećana,
a sadržaj MDA smanjen u odnosu na kontrolu. Korištenje ekotoksikoloških testova, uz
kemijsku analizu vode, omogućilo je detaljnu procjenu kvalitete vode rijeke Save i pružilo
mogućnost razdvajanja postaja na temelju stupnja onečišćenja.

Abstract (english)

Continuous industrial and technological development results in increasing number
of different contaminants present in the environment. Due to the possible negative
consequences that this numerous contaminants could induce, it is necessary to actively
monitor such environmental contamination. Two standardized bioassays were used in this
study to assess the water quality of the Sava River. Considering the important role of
invertebrates in freshwater environments, freshwater crustaceans Gammarus fossarum (Koch,
1836) and Daphnia magna (Straus, 1820) were used in this study as model organisms due to
their high sensitivity to water contamination, simple handling and easy assessment of
behavioral changes and changes in several biochemical parameters (detoxifying activity
followed by enzyme glutathione-S-transferase (GST), oxidative damage by measurement of
malondialdehyde (MDA) level) that can be measured on them. Model organisms have been
exposed to filtered water samples from five locations where different levels of water
contamination were established (Hrušćica > Rugvica > Jesenice > Lukavec > Jankomir). The
presence of contaminants at these locations was determined by high-throughput chemical
analysis of water. Post-exposure feeding test showed lower feeding rates, GST activity was
increased and the level of MDA was decreased when compared to control. The use of
ecotoxicological bioassays, combined with chemical analysis of water samples, has provided
a detailed assessment of the water quality of the Sava River and offered the possibility for
distinction of the locations based on the degree of water contamination.

• Keywords: procjena toksičnosti; kemijska karakterizacija vode; organska onečišćivala; biokemijski biomarkeri; kretanje životinja; Gammarus fossarum; Daphnia magna
• Keywords (english): toxicity assessment; chemical characterisation of water; organic contaminants; biochemical biomarkers; animal locomotion; Gammarus fossarum; Daphnia magna
• Language: croatian
• URN:NBN: urn:nbn:hr:217:721982
• Study programme: Title: Enviromental Sciences; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra struke znanosti o okolišu (magistar/magistra struke znanosti o okolišu)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2020-06-17
• Created on: 2019-12-02 13:52:02