Utjecaj pesticida karbendazima na preživljenje i populacijsku dinamiku vrste Euglena viridis

master's thesis

• Title (english): The impact of the pesticide carbendazim on the survival and population dynamics of Euglena viridis
• Author: Marija Gulan
• Mentor: Domagoj Đikić (mentor)
• Committee member: Domagoj Đikić (predsjednik povjerenstva)
• Committee member: Marko Miliša (član povjerenstva)
• Committee member: Antun Alegro (član povjerenstva)
• Committee member: Ana Galov (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2017-09-25, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Karbendazim je fungicid širokog spektra koji spada u skupinu benzimidazolnih spojeva i djeluje na način da inhibira diobu stanica. Postojan je i slabo topljiv u vodi te nakon tretiranja uzgajanih kultura zaostaje u hrani, predstavlja zdravstveni rizik za ljude i okoliš. Relativno su dobro poznati literaturni navodi o toksičnosti ovog pesticida i njegov utjecaj na ljudsko zdravlje. S obzirom da je utjecaj karbendazima na organizme vodenih ekosustava nedovoljno istražen, posebice na vrstama koje zauzimaju važnu ulogu u trofičkim lancima, cilj ovoga rada je istražiti potencijalno toksično djelovanje karbendazima na vrstu E. viridis. Čista kultura vrste tretirana je različitim rasponom doza karbendazima tijekom 18 dana pri čemu se pratila brojnost populacije. Pri akutnim koncentracijama, tijekom 24 sata karbendazim u koncentracijama od 0.1 nM do 100 nM ne uzrokuje smrtnost populacije, dok sve doze iznad 450 μM uzrokuju smrt kod 90–100 % euglena u populaciji. Utvrđena LC50 doza karbendazima za euglenu je 306,35 μM. Povećanje aktivnosti enzima SOD javlja se pri 200 μM koncentraciji u odnosu na kontrolnu grupu što je u skladu sa porastom intenziteta fluorescencijske boje DHE, koja ukazuje na blago povećanje razine superoksidnog aniona.

Abstract (english)

Carbendazim is a wide spectrum fungicide belonging to the group of benzimidazole compounds and its mechanism of acttion is inhibition of cell division. It is persistent and poorly soluble in water and after the treatment of cultured crops lags behind in food and can represents a health risk for humans and the environment. There are relatively well-known literary allegations about the toxicity of this pesticide and its impact on human health. Since the influence of carbendazim on aquatic organisms on aquatic organisms is insufficiently explored, especially on species that play an important role in trophic chains, the aim of this study is to investigate the potentially toxic effects of carbendazim on E. viridis. The pure culture was treated with a different range of carbendazim doses within 18 days, with the population count being monitored. At acute concentrations, within 24 hours carbendazine at concentrations of 0.1 nM to 100 nM does not cause population mortality, while all doses above 450 μM cause death in 90-100 % euglena in the population. Determined LC50 dose of carbendazole for eugenas is 306,35 μM. Increasing activity of SOD enzymes occurs at 200 μM concentration in relation to the control group, which is consistent with the increase in intensity of the fluorescence dye DHE, which indicates a slight increase in the level of the superoxide anion.

• Keywords: karbendazim; Euglena viridis; toksičnost; populacijska dinamika
• Keywords (english): carbendazim; Euglena viridis; toxicity; population dynamics
• Language: croatian
• URN:NBN: urn:nbn:hr:217:136234
• Study programme: Title: Ecology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra ekologije i zaštite prirode (magistar/magistra ekologije i zaštite prirode)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2019-09-25
• Created on: 2017-10-20 11:24:15