Izolacija i karakterizacija bakterije Pseudomonas aeruginosa iz uzoraka vode

master's thesis

• Title (english): Isolation and characterization of bacterium Pseudomonas aeruginosa from water samples
• Author: Doris Tafra
• Mentor: Ana Kovačić (mentor)
• Mentor: Jasna Hrenović (mentor)
• Committee member: Jasna Hrenović (predsjednik povjerenstva)
• Committee member: Sven Jelaska (član povjerenstva)
• Committee member: Ana Galov (član povjerenstva)
• Committee member: Jasna Lajtner (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2017-11-15, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Bakterija Pseudomonas aeruginosa je Gram-negativni aerobni štapić. Humani je patogen koji je široko rasprostranjen u prirodi. Nazivamo ga oportunističkim patogenom jer izaziva bolesti samo kod ljudi s oslabljenim imunitetom. Lako se širi u vlažnom okolišu i stvara biofilm te je upravo zato važno znati više o njegovoj otpornosti i preživljavanju u različitim uvjetima. P. aeruginosa su izdvojeni i biokemijski potvrĎeni iz različitih uzoraka vode. Za 10 odabranih izolata praćeno je preživljavanje u sterilnoj vodi kroz 28 dana. Svi su izolati preživjeli tijekom ispitivanog perioda. Najveće preživljenje je imao izolat P. aeruginosa izdvojen iz vode brodskog tanka, a najmanje izolat izdvojen iz uzorka boćate vode rijeke Cetine. Ostali izolati su pokazali ujednačeno preživljenje. P. aeruginosa posjeduje različite mehanizme koji joj omogućuju preživljenje u nepovoljnim životnim uvjetima, pogotovo onima kod manjka nutrijenata.

Abstract (english)

Bacteria P. aeruginosa is a Gram-negative aerobic bacillus. It`s a human pathogen that is widespread in nature. It`s called an opportunistic pathogen because it causes disease only in people with imparied immunity. P. aeruginosa spreads easily in humid environment and has elaborate mechanisms for adhering to solid surfaces and establishing biofilms so it`s important to find out more about its resistance and survival in different conditions. P. aeruginosa was isolated and biochemically confirmed from different water samples. We selected 10 isolates and monitored their survival in sterile water over a period of 28 days. All isolates survived during the examined period. P. aeruginosa from ship`s tank survived the longest, and the shortest survival had P. aeruginosa from brackish water from river Cetina. Other isolates showed even survival rate. P. aeruginosa has various mechanisms that helps her to survive in different conditions , especially those with a lack of nutrition.

• Keywords: patogen; biofilm; preživljenje; izolat; nutrijenti
• Keywords (english): pathogen; biofilm; survival; isolate; nutrition
• Language: croatian
• URN:NBN: urn:nbn:hr:217:959858
• Study programme: Title: Ecology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra ekologije i zaštite prirode (magistar/magistra ekologije i zaštite prirode)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-12-07 13:13:17