Identifikacija i karakterizacija citokroma P450 obitelji 4 u dagnji, Mytilus galloprovincialis

doctoral thesis

• Title (english): Identification and characterization of cytochrome P450 family 4 in the mussel, Mytilus galloprovincialis
• Author: Sanda Ravlić; MBZ: 305793
• Mentor: Maja Fafanđel (mentor); MBZ: 218864
• Committee member: Nevenka Bihari (predsjednik povjerenstva)
• Committee member: Tvrtko Smital (član povjerenstva)
• Committee member: Goran Klobučar (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Geology); Zagreb
• Defense date and country: 2014-07-15, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Interdisciplinary Natural Sciences; Marine Science
• Universal decimal classification (UDC): 55 - Geology. Meteorology. Hydrology

Abstract

Enzimi citokroma P450 (CYP) čine važnu komponentu staničnog sustava detoksifikacije. U dagnji, Mytilus galloprovincialis, s različitih lokacija, identificirano je i karakterizirano sedam novih eksprimiranih grupa gena citokroma P450. Filogenetska analiza enzima citokroma P450 pokazala je grupiranje eksprimiranih gena unutar CYP4 monofiletske grupe. Usporedba prevedenih CYP4 aminokiselinskih sljedova dagnje sa CYP4 sljedovima kralješnjaka i beskralješnjaka pokazala je visoki stupanj očuvanja aminokiselina u domenama bitnim za funkcionalnost enzima. Identificirane genske grupe, od kojih svaka sadrži niz genskih varijanti, imenovane su CYP4Y1, Y2, Y3, Y4, Y5, Y6 i Y7. Grupe gena CYP4Y2 i Y7 te CYP4Y5 i Y6 pokazale su mjesnu specifičnost, dok je prisutnost genskih grupa CYP4Y1, Y3 i Y4 uočena na svim lokacijama uzorkovanja. Dobiveni rezultati ukazuju na veliku ekspanziju genske obitelji CYP4Y u dagnji, vjerojatno kao posljedicu različitih uvjeta u okolišu. Podatci koji se odnose na raznovrsnost gena cyp4y mogli bi predstavljati dobru osnovu za buduća istraživanja usmjerena na poboljšanje primjene citokroma P450 kao biomarkera onečišćenja okoliša.

Abstract (english)

Cytochrome P450 enzymes (CYPs) are essential components of cellular detoxification system. In this study seven new cytochrome P450 gene clusters from bivalve mollusc Mytilus galloprovincialis from different collection sites were identified and characterized. The phylogenetic analysis demonstrated that the identified sequences were clustered within the CYP4 branch. The deduced amino acid sequences showed high conservation of the residues and domains essential to the function of the enzyme. Identified gene transcripts corresponded to seven different gene clusters, each comprising a number of allelic variants, which were designated CYP4Y1, Y2, Y3, Y4, Y5, Y6 and Y7. Gene clusters CYP4Y2 and Y7, and CYP4Y5 and Y6 were site specific, while the gene clusters CYP4Y1, Y3 and Y4 were present at all investigated locations. Great expansion of the CYP4Y gene subfamily in the mussel M. galloprovincialis could be attributed to different environmental conditions. Data concerning diversity of CYP4Y genes could present a good base in further investigations for improvement of cytochrome P450 application as biomarker of marine environmental pollution.

• Keywords: citokrom P450; cDNA kloniranje; filogenetska analiza; okoliš; školjkaši
• Keywords (english): cytochrome P450; cDNA cloning; phylogenetic analysis; environment; bivalvia
• Language: croatian
• URN:NBN: urn:nbn:hr:217:510934
• Study programme: Title: Interdisciplinary doctoral study in Oceanology; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje interdisciplinarne prirodne znanosti (znanost o moru) (doktor/doktorica znanosti, područje prirodnih znanosti, polje interdisciplinarne prirodne znanosti (znanost o moru))
• Type of resource: Text
• Extent: 112 (+XII) str. ; 30 cm
• File origin: Born digital
• Access conditions: Open access
• Public note: Doktorska disertacija izrađena je u Centru za istraživanje mora Instituta Ruđer Bošković u Rovinju, u sklopu navedenog studija.
• Created on: 2017-02-17 11:33:01